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Anti-ATP5A1 Rabbit Monoclonal Antibody #1896

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Flow cytometric analysis of ATP5A1 expression in HepG2 cells using ATP5A1 antibody (Cat#1896, 1:2,000). Green, isotype control; red, ATP5A1.

Immunocytochemical staining of HepG2 cells with anti-ATP5A1 antibody (Cat #1896, 1:1,000) . Nuclei were stained blue with DAPI; ATP5A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-ATP5A1 antibody (Cat#1896). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP5A1 antibody (Cat#1896, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

ATP5F1A
货号：

1896
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Citation(0)

Information

Product Name	Anti-ATP5A1 Rabbit Monoclonal Antibody
Aliases	ATP5F1A; ATP Synthase F1 Subunit Alpha; ATP5A; ATP5AL2; ATP5A1; HATP1; ATPM; OMR; ORM; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Alpha Subunit 1, Cardiac Muscle; ATP Synthase Subunit Alpha, Mitochondrial; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Alpha Subunit, Isoform 2, Non-Cardiac Muscle-Like 2; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Alpha Subunit, Isoform 1, Cardiac Muscle; Epididymis Secretory Sperm Binding Protein Li 123m; Mitochondrial ATP Synthetase, Oligomycin-Resistant; ATP Synthase Alpha Chain, Mitochondrial; ATP Sythase (F1-ATPase) Alpha Subunit; EC 3.6.3.14; HEL-S-123m; EC 3.6.3; COXPD22; MC5DN4A; MC5DN4B; MC5DN4; MOM2
Background	Gene Name: ATP5F1A NCBI Gene Entry: 498 UniProt Entry: P25705
Application Information	Molecular Weight: Predicted, 60 kDa; observed, 54 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB4850 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human ATP5A1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of ATP5A1 expression in HepG2 cells using ATP5A1 antibody (Cat#1896, 1:2,000). Green, isotype control; red, ATP5A1.
Immunocytochemical staining of HepG2 cells with anti-ATP5A1 antibody (Cat #1896, 1:1,000) . Nuclei were stained blue with DAPI; ATP5A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ATP5A1 antibody (Cat#1896). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP5A1 antibody (Cat#1896, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

ATP5F1A
货号：

1896
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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