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Anti-DDB2 Rabbit Monoclonal Antibody #4614

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Flow cytometric analysis of DDB2 expression in HT-1080 cells using anti-DDB2 antibody (Cat#4614, 1:2,000). Green, isotype control; red, DDB2.
Immunocytochemical staining of HT-1080 cells with anti-DDB2 antibody (Cat#4614, 1:1,000) . Nuclei were stained blue with DAPI; DDB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-DDB2 antibody (Cat#4614). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDB2 antibody (Cat#4614, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    DDB2
  • 货号:
    4614
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-DDB2 Rabbit Monoclonal Antibody
Aliases DDB2; Damage Specific DNA Binding Protein 2; UV-Damaged DNA-Binding Protein 2; DDB P48 Subunit; UV-DDB2; DDBB; XPE; Xeroderma Pigmentosum Group E Protein; DNA Damage-Binding Protein 2; FLJ34321; Damage-Specific DNA Binding Protein 2 (48kD); Damage-Specific DNA Binding Protein 2, 48kDa; Damage-Specific DNA-Binding Protein 2; UV-DDB; DDBb
Background

Gene Name: DDB2

NCBI Gene Entry: 1643

UniProt Entry: Q92466

Application Information

Molecular Weight: Predicted, 48 kDa; observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12080

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human DDB2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of DDB2 expression in HT-1080 cells using anti-DDB2 antibody (Cat#4614, 1:2,000). Green, isotype control; red, DDB2.
  • Immunocytochemical staining of HT-1080 cells with anti-DDB2 antibody (Cat#4614, 1:1,000) . Nuclei were stained blue with DAPI; DDB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-DDB2 antibody (Cat#4614). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDB2 antibody (Cat#4614, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of DDB2 expression in HT-1080 cells using anti-DDB2 antibody (Cat#4614, 1:2,000). Green, isotype control; red, DDB2.
Immunocytochemical staining of HT-1080 cells with anti-DDB2 antibody (Cat#4614, 1:1,000) . Nuclei were stained blue with DAPI; DDB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-DDB2 antibody (Cat#4614). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDB2 antibody (Cat#4614, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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