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Anti-pan Cadherin Rabbit Monoclonal Antibody #1904

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Flow cytometric analysis of pan Cadherin expression in HepG2 cells using pan Cadherin antibody (Cat#1904, 1:2,000). Green, isotype control; red, pan Cadherin.
Immunocytochemical staining of HepG2 cells with anti-pan Cadherin antibody (Cat#1904, 1:1,000). Nuclei were stained blue with DAPI; pan Cadherin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-pan Cadherin antibody (Cat#1904). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-pan Cadherin antibody (Cat#1904, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CDH1/2/3/5/4
  • 货号:
    1904
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-pan Cadherin Rabbit Monoclonal Antibody
Aliases CDH1; Cadherin 1; Uvomorulin; CD324; UVO; Cadherin 1, Type 1, E-Cadherin (Epithelial); Epithelial Cadherin; E-Cadherin; Cadherin-1; CAM 120/80; CDHE; Calcium-Dependent Adhesion Protein, Epithelial; Epididymis Secretory Sperm Binding Protein; Cadherin 1, E-Cadherin (Epithelial); Cell-CAM 120/80; CD324 Antigen; E-Cadherin 1; UVOMORULIN; Arc-1; BCDS1; ECAD; LCAM
Background

Gene Name: CDH1/2/3/5/4

NCBI Gene Entry: 999/1000/1001/1003/1002

UniProt Entry: P12830/P19022/P22223/P33151/P55283

Application Information

Molecular Weight: Predicted, 100 kDa; observed, 140 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5050

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human pan Cadherin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of pan Cadherin expression in HepG2 cells using pan Cadherin antibody (Cat#1904, 1:2,000). Green, isotype control; red, pan Cadherin.
  • Immunocytochemical staining of HepG2 cells with anti-pan Cadherin antibody (Cat#1904, 1:1,000). Nuclei were stained blue with DAPI; pan Cadherin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-pan Cadherin antibody (Cat#1904). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-pan Cadherin antibody (Cat#1904, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of pan Cadherin expression in HepG2 cells using pan Cadherin antibody (Cat#1904, 1:2,000). Green, isotype control; red, pan Cadherin.
Immunocytochemical staining of HepG2 cells with anti-pan Cadherin antibody (Cat#1904, 1:1,000). Nuclei were stained blue with DAPI; pan Cadherin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-pan Cadherin antibody (Cat#1904). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-pan Cadherin antibody (Cat#1904, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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