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Anti-VPS24 Rabbit Monoclonal Antibody #1910

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Flow cytometric analysis of VPS24 expression in C2C12 cells using VPS24 antibody (Cat#1910, 1:2,000). Green, isotype control; red, VPS24.
Immunocytochemical staining of C2C12 cells with anti-VPS24 antibody (Cat #1910, 1:1,000) . Nuclei were stained blue with DAPI; VPS24 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-VPS24 antibody (Cat#1910). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-VPS24 antibody (Cat#1910, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CHMP3
  • 货号:
    1910
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-VPS24 Rabbit Monoclonal Antibody
Aliases CHMP3; Charged Multivesicular Body Protein 3; NEDF; Neuroendocrine Differentiation Factor; CGI-149; VPS24; Vacuolar Protein Sorting-Associated Protein 24; Chromatin-Modifying Protein 3; Vacuolar Protein Sorting 24 Homolog (S. Cerevisiae); Vacuolar Protein Sorting 24 (Yeast); Vacuolar Protein Sorting 24 Homolog; CHMP Family, Member 3; 25.1 Protein; CGI149; HVps24
Background

Gene Name: CHMP3

NCBI Gene Entry: 51652

UniProt Entry: Q9Y3E7

Application Information

Molecular Weight: Predicted, 25 kDa; observed, 29 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5080

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human VPS24
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of VPS24 expression in C2C12 cells using VPS24 antibody (Cat#1910, 1:2,000). Green, isotype control; red, VPS24.
  • Immunocytochemical staining of C2C12 cells with anti-VPS24 antibody (Cat #1910, 1:1,000) . Nuclei were stained blue with DAPI; VPS24 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-VPS24 antibody (Cat#1910). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-VPS24 antibody (Cat#1910, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of VPS24 expression in C2C12 cells using VPS24 antibody (Cat#1910, 1:2,000). Green, isotype control; red, VPS24.
Immunocytochemical staining of C2C12 cells with anti-VPS24 antibody (Cat #1910, 1:1,000) . Nuclei were stained blue with DAPI; VPS24 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-VPS24 antibody (Cat#1910). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-VPS24 antibody (Cat#1910, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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