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Anti-Chymase 1 Rabbit Monoclonal Antibody #1913

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Flow cytometric analysis of Chymase 1 expression in C2C12 cells using anti-Chymase 1 antibody (Cat#1913, 1:2,000). Green, isotype control; red, Chymase 1.
Immunocytochemical staining of C2C12 cells with anti-Chymase 1 antibody (Cat#1913, 1:1,000) . Nuclei were stained blue with DAPI;Chymase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-Chymase 1 antibody (Cat#1913). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Chymase 1 antibody (Cat#1913, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    CMA1
  • 货号：
    1913
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    M
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Chymase 1 Rabbit Monoclonal Antibody
Aliases CMA1; Chymase 1; Chymase 1, Mast Cell; Mast Cell Protease I; Alpha-Chymase; EC 3.4.21.39; Chymase; CYH; Chymase 1 Preproprotein Transcript E; Chymase 1 Preproprotein Transcript I; Chymase, Mast Cell; Chymase, Heart; EC 3.4.21; MCT1; CYM
Background

Gene Name: CMA1

NCBI Gene Entry: 1215

UniProt Entry: P23946
Application Information

Molecular Weight: Predicted, 27 kDa; observed, 27 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5095

Species Reactivity: Mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Mast Cell Chymase
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM) : 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Chymase 1 expression in C2C12 cells using anti-Chymase 1 antibody (Cat#1913, 1:2,000). Green, isotype control; red, Chymase 1.
  • Immunocytochemical staining of C2C12 cells with anti-Chymase 1 antibody (Cat#1913, 1:1,000) . Nuclei were stained blue with DAPI;Chymase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-Chymase 1 antibody (Cat#1913). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Chymase 1 antibody (Cat#1913, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    CMA1
  • 货号：
    1913
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    M
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Chymase 1 expression in C2C12 cells using anti-Chymase 1 antibody (Cat#1913, 1:2,000). Green, isotype control; red, Chymase 1.
Immunocytochemical staining of C2C12 cells with anti-Chymase 1 antibody (Cat#1913, 1:1,000) . Nuclei were stained blue with DAPI;Chymase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-Chymase 1 antibody (Cat#1913). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Chymase 1 antibody (Cat#1913, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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