Flow cytometric analysis of SATB2 expression in HeLa cells using anti-SATB2 antibody (Cat#3131, 1:2,000). Green, isotype control; red, SATB2.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using anti-SATB2 antibody (Cat#3131, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-SATB2 antibody (Cat#3131). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SATB2 antibody (Cat#3131, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
SATB2; SATB Homeobox 2; KIAA1034; Special AT-Rich Sequence-Binding Protein 2; DNA-Binding Protein SATB2; SATB Family Member 2; FLJ21474; SATB2 Fusion; C2DELq32q33; DEL2Q32Q33; GLSS
Flow cytometric analysis of SATB2 expression in HeLa cells using anti-SATB2 antibody (Cat#3131, 1:2,000). Green, isotype control; red, SATB2.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using anti-SATB2 antibody (Cat#3131, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-SATB2 antibody (Cat#3131). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SATB2 antibody (Cat#3131, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of SATB2 expression in HeLa cells using anti-SATB2 antibody (Cat#3131, 1:2,000). Green, isotype control; red, SATB2.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using anti-SATB2 antibody (Cat#3131, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-SATB2 antibody (Cat#3131). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SATB2 antibody (Cat#3131, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
