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Anti-CYBB Rabbit Monoclonal Antibody #1921

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Flow cytometric analysis of CYBB expression in HT-1080 cells using CYBB antibody (Cat#1921, 1:2,000). Green, isotype control; red, CYBB.

Immunocytochemical staining of HT-1080 cells with anti-CYBB antibody (Cat #1921, 1:1,000) . Nuclei were stained blue with DAPI; CYBB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-CYBB antibody (Cat#1921). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CYBB antibody (Cat#1921, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CYBB
货号：

1921
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-CYBB Rabbit Monoclonal Antibody
Aliases	CYBB; Cytochrome B-245 Beta Chain; NOX2; Cytochrome B-245 Heavy Chain; Cytochrome B558 Subunit Beta; NADPH Oxidase 2; GP91-PHOX; Superoxide-Generating NADPH Oxidase Heavy Chain Subunit; Heme-Binding Membrane Glycoprotein Gp91phox; Neutrophil Cytochrome B 91 KDa Polypeptide; Cytochrome B(558) Subunit Beta; P22 Phagocyte B-Cytochrome; CGD91-Phox; GP91PHOX; P91-PHOX; CGD; Cytochrome B-245, Beta Polypeptide; Cytochrome B-245 Beta Polypeptide; Chronic Granulomatous Disease; EC 1.-.-.-; Gp91-Phox; EC 1.6.3; AMCBX2; GP91-1; Gp91-1; IMD34; CGDX
Background	Gene Name: CYBB NCBI Gene Entry: 1536 UniProt Entry: P04839
Application Information	Molecular Weight: Predicted, 65 kDa; observed, 65 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5135 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human NOX2/CYBB/gp91phox
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of CYBB expression in HT-1080 cells using CYBB antibody (Cat#1921, 1:2,000). Green, isotype control; red, CYBB.
Immunocytochemical staining of HT-1080 cells with anti-CYBB antibody (Cat #1921, 1:1,000) . Nuclei were stained blue with DAPI; CYBB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CYBB antibody (Cat#1921). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CYBB antibody (Cat#1921, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CYBB
货号：

1921
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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