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Anti-E2F6 Rabbit Monoclonal Antibody #1948

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Flow cytometric analysis of E2F6 expression in HeLa cells using anti-E2F6 antibody (Cat#1948, 1:2,000). Green, isotype control; red, E2F6.
Immunocytochemical staining of Hela cells with anti-E2F6 antibody (Cat #1948, 1:1,000) . Nuclei were stained blue with DAPI; E2F6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-E2F6 antibody (Cat#1948). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-E2F6 antibody (Cat#1948, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    E2F6
  • 货号：
    1948
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-E2F6 Rabbit Monoclonal Antibody
Aliases E2F6; E2F Transcription Factor 6; E2F-6; Transcription Factor E2F6
Background

Gene Name: E2F6

NCBI Gene Entry: 1876

UniProt Entry: O75461
Application Information

Molecular Weight: Predicted, 32 kDa; observed, 35 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5255

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human E2F6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
IImmunohistochemistry (IHC): 1:100-1:1,000
Flow Cytometry (FCM) : 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of E2F6 expression in HeLa cells using anti-E2F6 antibody (Cat#1948, 1:2,000). Green, isotype control; red, E2F6.
  • Immunocytochemical staining of Hela cells with anti-E2F6 antibody (Cat #1948, 1:1,000) . Nuclei were stained blue with DAPI; E2F6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-E2F6 antibody (Cat#1948). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-E2F6 antibody (Cat#1948, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    E2F6
  • 货号：
    1948
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of E2F6 expression in HeLa cells using anti-E2F6 antibody (Cat#1948, 1:2,000). Green, isotype control; red, E2F6.
Immunocytochemical staining of Hela cells with anti-E2F6 antibody (Cat #1948, 1:1,000) . Nuclei were stained blue with DAPI; E2F6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-E2F6 antibody (Cat#1948). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-E2F6 antibody (Cat#1948, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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