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Anti-EIF2B5 Rabbit Monoclonal Antibody #1957

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Flow cytometric analysis of EIF2B5 expression in HepG2 cells using EIF2B5 antibody (Cat#1957, 1:2,000). Green, isotype control; red, EIF2B5.

Immunocytochemical staining of HepG2 cells with anti-EIF2B5 antibody (Cat#1957, 1:1000) . Nuclei were stained blue with DAPI; EIF2B5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-EIF2B5 antibody (Cat#1957). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF2B5 antibody (Cat#1957, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

EIF2B5
货号：

1957
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-EIF2B5 Rabbit Monoclonal Antibody
Aliases	EIF2B5; Eukaryotic Translation Initiation Factor 2B Subunit Epsilon; EIF-2B; Eukaryotic Translation Initiation Factor 2B, Subunit 5 Epsilon, 82kDa; Translation Initiation Factor EIF-2B Subunit Epsilon; EIF-2B GDP-GTP Exchange Factor Subunit Epsilon; EIF2Bepsilon; Eukaryotic Translation Initiation Factor 2B, Subunit 5 (Epsilon, 82kD); EIF2BEPSILON; EIF2BE; CACH; LVWM; VWM5; CLE
Background	Gene Name: EIF2B5 NCBI Gene Entry: 8893 UniProt Entry: Q13144
Application Information	Molecular Weight: Predicted, 80 kDa; observed, 80 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5300 Species Reactivity: Human Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human eIF2B epsilon
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of EIF2B5 expression in HepG2 cells using EIF2B5 antibody (Cat#1957, 1:2,000). Green, isotype control; red, EIF2B5.
Immunocytochemical staining of HepG2 cells with anti-EIF2B5 antibody (Cat#1957, 1:1000) . Nuclei were stained blue with DAPI; EIF2B5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-EIF2B5 antibody (Cat#1957). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF2B5 antibody (Cat#1957, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

EIF2B5
货号：

1957
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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