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Anti-BubR1 Rabbit Monoclonal Antibody #4327

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Flow cytometric analysis of BubR1 expression in HepG2 cells using anti-BubR1 antibody (Cat#4327, 1:2,000). Green, isotype control; red, BubR1.

Immunocytochemical staining of HepG2 cells with anti-BubR1 antibody (Cat#4327, 1:1,000) . Nuclei were stained blue with DAPI; BubR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-BubR1 antibody (Cat#4327). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BubR1 antibody (Cat#4327, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

BUB1B
货号：

4327
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-BubR1 Rabbit Monoclonal Antibody
Aliases	BUB1 Mitotic Checkpoint Serine/Threonine Kinase B; BUBR1; MAD3L; SSK1; Bub1A; Mitotic Checkpoint Serine/Threonine-Protein Kinase BUB1 Beta; MAD3/BUB1-Related Protein Kinase; Mitotic Checkpoint Kinase MAD3L; HBUBR1; Budding Uninhibited By Benzimidazoles 1 (Yeast Homolog), Beta; Budding Uninhibited By Benzimidazoles 1 Homolog Beta (Yeast); Budding Uninhibited By Benzimidazoles 1 Homolog Beta; BUB1B, Mitotic Checkpoint Serine/Threonine Kinase; Protein SSK1; EC 2.7.11.1; BUB1beta; MVA1
Background	Gene Name: BUB1B NCBI Gene Entry: 701 UniProt Entry: O60566
Application Information	Molecular Weight: Predicted, 120 kDa; observed, 120 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10665 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human BubR1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of BubR1 expression in HepG2 cells using anti-BubR1 antibody (Cat#4327, 1:2,000). Green, isotype control; red, BubR1.
Immunocytochemical staining of HepG2 cells with anti-BubR1 antibody (Cat#4327, 1:1,000) . Nuclei were stained blue with DAPI; BubR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-BubR1 antibody (Cat#4327). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BubR1 antibody (Cat#4327, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

BUB1B
货号：

4327
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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