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Anti-GNE Rabbit Monoclonal Antibody #2012

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Flow cytometric analysis of GNE expression in HeLa cells using anti-GNE antibody (Cat#2012, 1：2000). Green, isotype control; red, GNE.

Immunocytochemical staining of HeLa cells with anti-GNE antibody (Cat#2012, 1:1,000) . Nuclei were stained blue with DAPI; GNE was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-GNE antibody (Cat#2012). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GNE antibody (Cat#2012, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

GNE
货号：

2012
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-GNE Rabbit Monoclonal Antibody
Aliases	GNE; Glucosamine (UDP-N-Acetyl)-2-Epimerase/N-Acetylmannosamine Kinase; Bifunctional UDP-N-Acetylglucosamine 2; Epimerase/N-Acetylmannosamine Kinase; UDP-GlcNAc-2-Epimerase/ManAc Kinase; GLCNE; Uae1; IBM2; UDP-N-Acetylglucosamine-2-Epimerase/N-Acetylmannosamine Kinase; UDP-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase; N-Acylmannosamine Kinase; DMRV; UAE1; NM
Background	Gene Name: GNE NCBI Gene Entry: 10020 UniProt Entry: Q9Y223
Application Information	Molecular Weight: Predicted, 79 kDa; observed, 75 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5560 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human GNE
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of GNE expression in HeLa cells using anti-GNE antibody (Cat#2012, 1：2000). Green, isotype control; red, GNE.
Immunocytochemical staining of HeLa cells with anti-GNE antibody (Cat#2012, 1:1,000) . Nuclei were stained blue with DAPI; GNE was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-GNE antibody (Cat#2012). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GNE antibody (Cat#2012, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

GNE
货号：

2012
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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