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Anti-P2Y12 Rabbit Monoclonal Antibody #2035

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Flow cytometric analysis of P2Y12 expression in HepG2 cells using P2Y12 antibody (Cat#2035, 1:2,000). Green, isotype control; red, P2Y12.

Immunocytochemical staining of HepG2 cells with anti-P2Y12 antibody (Cat#2035, 1:1,000). Nuclei were stained blue with DAPI; P2Y12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-P2Y12 antibody (Cat#2035). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-P2Y12 antibody (Cat#2035, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

P2RY12
货号：

2035
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-P2Y12 Rabbit Monoclonal Antibody
Aliases	P2RY12; Purinergic Receptor P2Y12; SP1999; P2Y12; HORK3; Purinergic Receptor P2Y, G-Protein Coupled, 12; P2Y12 Platelet ADP Receptor; ADP-Glucose Receptor; P2Y Purinoceptor 12; P2Y(ADP); P2Y(Cyc); P2T(AC); P2Y(AC); ADPG-R; Putative G-Protein Coupled Receptor; G-Protein Coupled Receptor SP1999; Gi-Coupled ADP Receptor HORK3; Purinergic Receptor P2RY12; P2Y(12)R; BDPLT8
Background	Gene Name: P2RY12 NCBI Gene Entry: 64805 UniProt Entry: Q9H244
Application Information	Molecular Weight: Predicted, 39 kDa; observed, 50 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB4970 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human P2Y12
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of P2Y12 expression in HepG2 cells using P2Y12 antibody (Cat#2035, 1:2,000). Green, isotype control; red, P2Y12.
Immunocytochemical staining of HepG2 cells with anti-P2Y12 antibody (Cat#2035, 1:1,000). Nuclei were stained blue with DAPI; P2Y12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-P2Y12 antibody (Cat#2035). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-P2Y12 antibody (Cat#2035, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

P2RY12
货号：

2035
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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