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KD-Validated Anti-S1PR3 Rabbit Monoclonal Antibody #61530

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Flow cytometric analysis of S1PR3 expression in HAP-1 cells using S1PR3 antibody (Cat#61530, 1:2,000). Green, isotype control; red, S1PR3.
Immunocytochemical staining of HAP1 cells with anti-S1PR3 antibody (Cat#61530, 1:1,000). Nuclei were stained blue with DAPI; S1PR3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-S1PR3 antibody (Cat#61530). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-S1PR3 antibody (Cat#61530, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-S1PR3 antibody (Cat#61530). S1PR3 expression in wild-type (WT) and S1PR3 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-S1PR3 antibody (Cat#61530, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    S1PR3
  • 货号：
    61530
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-S1PR3 Rabbit Monoclonal Antibody
Aliases S1PR3; Sphingosine-1-Phosphate Receptor 3; BA791O21.3; C9orf108; C9orf47; EDG-3; EDG3; Endothelial Differentiation, Sphingolipid G-Protein-Coupled Receptor,3; Endothelial Differentiation G-Protein Coupled Receptor 3; Sphingosine 1-Phosphate Receptor Edg-3; Sphingosine 1-Phosphate Receptor 3; S1P Receptor Edg-3; S1P Receptor 3; FLJ37523; S1P3; G Protein-Coupled Receptor, Endothelial Differentiation Gene-3; Chromosome 9 Open Reading Frame 108; Chromosome 9 Open Reading Frame 47; Uncharacterized Protein C9orf47; S1P Receptor EDG3; LPB3
Background

Gene Name: S1PR3

NCBI Gene Entry: 1903

UniProt Entry: Q99500
Application Information

Molecular Weight: Predicted, 42 kDa; observed, 42 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2660

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human S1PR3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of S1PR3 expression in HAP-1 cells using S1PR3 antibody (Cat#61530, 1:2,000). Green, isotype control; red, S1PR3.
  • Immunocytochemical staining of HAP1 cells with anti-S1PR3 antibody (Cat#61530, 1:1,000). Nuclei were stained blue with DAPI; S1PR3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-S1PR3 antibody (Cat#61530). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-S1PR3 antibody (Cat#61530, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-S1PR3 antibody (Cat#61530). S1PR3 expression in wild-type (WT) and S1PR3 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-S1PR3 antibody (Cat#61530, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    S1PR3
  • 货号：
    61530
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of S1PR3 expression in HAP-1 cells using S1PR3 antibody (Cat#61530, 1:2,000). Green, isotype control; red, S1PR3.
Immunocytochemical staining of HAP1 cells with anti-S1PR3 antibody (Cat#61530, 1:1,000). Nuclei were stained blue with DAPI; S1PR3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-S1PR3 antibody (Cat#61530). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-S1PR3 antibody (Cat#61530, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-S1PR3 antibody (Cat#61530). S1PR3 expression in wild-type (WT) and S1PR3 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-S1PR3 antibody (Cat#61530, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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