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Anti-Estrogen receptor 2 Rabbit Monoclonal Antibody #2051

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Flow cytometric analysis of Estrogen receptor 2 expression in HeLa cells using Estrogen receptor 2 antibody (Cat#2051, 1:2,000). Green, isotype control; red, Estrogen receptor 2.
Immunocytochemical staining of Hela cells with anti-Estrogen receptor 2 antibody (Cat#2051, 1:1,000). Nuclei were stained blue with DAPI; Estrogen receptor 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Estrogen receptor 2 antibody (Cat#2051). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Estrogen receptor 2 antibody (Cat#2051, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    ESR2
  • 货号:
    2051
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Estrogen receptor 2 Rabbit Monoclonal Antibody
Aliases ESR2; Estrogen Receptor 2; NR3A2; Nuclear Receptor Subfamily 3 Group A Member 2; Estrogen Receptor Beta; Oestrogen Receptor Beta; ER-Beta; ER-BETA; ESTRB; Erb; Estrogen Receptor 2 (ER Beta); Estrogen Receptor Beta 2; Estrogen Receptor Beta 4; ESR-BETA; ER Beta; ESRB; ODG8; ERB
Background

Gene Name: ESR2

NCBI Gene Entry: 2100

UniProt Entry: Q92731

Application Information

Molecular Weight: Predicted, 59 kDa; observed, 59 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5755

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Estrogen Receptor beta
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Estrogen receptor 2 expression in HeLa cells using Estrogen receptor 2 antibody (Cat#2051, 1:2,000). Green, isotype control; red, Estrogen receptor 2.
  • Immunocytochemical staining of Hela cells with anti-Estrogen receptor 2 antibody (Cat#2051, 1:1,000). Nuclei were stained blue with DAPI; Estrogen receptor 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Estrogen receptor 2 antibody (Cat#2051). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Estrogen receptor 2 antibody (Cat#2051, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Estrogen receptor 2 expression in HeLa cells using Estrogen receptor 2 antibody (Cat#2051, 1:2,000). Green, isotype control; red, Estrogen receptor 2.
Immunocytochemical staining of Hela cells with anti-Estrogen receptor 2 antibody (Cat#2051, 1:1,000). Nuclei were stained blue with DAPI; Estrogen receptor 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Estrogen receptor 2 antibody (Cat#2051). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Estrogen receptor 2 antibody (Cat#2051, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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