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Anti-Aromatase Rabbit Monoclonal Antibody #2054

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Flow cytometric analysis of Aromatase expression in HepG2 cells using Aromatase antibody (Cat#2054, 1:2,000). Green, isotype control; red, Aromatase.

Immunocytochemical staining of HepG2 cells with anti-Aromatase antibody (Cat#2054, 1:1,000). Nuclei were stained blue with DAPI; Aromatase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Aromatase antibody (Cat#2054). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aromatase antibody (Cat#2054, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CYP19A1
货号：

2054
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Aromatase Rabbit Monoclonal Antibody
Aliases	CYP19A1; Cytochrome P450 Family 19 Subfamily A Member; ARO; CYAR; P-450AROM; Aromatase; CYP19; CPV1; ARO; Cytochrome P450, Subfamily XIX (Aromatization Of Androgens); Cytochrome P450, Family 19, Subfamily A, Polypeptide 1; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen Synthase; CYPXIX; Flavoprotein-Linked Monooxygenase; Microsomal Monooxygenase; Estrogen Synthetase; EC 1.14.14.14; EC 1.14.14.1; AROMATASE
Background	Gene Name: CYP19A1 NCBI Gene Entry: 1588 UniProt Entry: P11511
Application Information	Molecular Weight: Predicted, 58 kDa; observed, 53 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5770 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Aromatase
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Aromatase expression in HepG2 cells using Aromatase antibody (Cat#2054, 1:2,000). Green, isotype control; red, Aromatase.
Immunocytochemical staining of HepG2 cells with anti-Aromatase antibody (Cat#2054, 1:1,000). Nuclei were stained blue with DAPI; Aromatase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Aromatase antibody (Cat#2054). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aromatase antibody (Cat#2054, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CYP19A1
货号：

2054
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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