Flow cytometric analysis of ILF2 expression in HAP-1 cells using anti-ILF2 antibody (1：2000). Green, isotype control; red, ILF2.
Western blotting analysis using anti-HIBCH antibody (Cat#64807). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIBCH antibody (Cat#64807, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-HIBCH antibody (Cat#64807). HIBCH expression in wild-type (WT) and HIBCH shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HIBCH antibody (Cat#64807, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of HIBCH knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-HIBCH antibody (Cat#64807, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-HIBCH antibody (Cat#64807, 1:1,000), Top panel: wild-type (WT); Bottom panal: HIBCH shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
