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Anti-FAM50A Rabbit Monoclonal Antibody #2066

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Flow cytometric analysis of FAM50A expression in HepG2 cells using FAM50A antibody (Cat#2066, 1:2,000). Green, isotype control; red, FAM50A.
Immunocytochemical staining of HepG2 cells with anti-FAM50A antibody (Cat#2066, 1:1,000). Nuclei were stained blue with DAPI; FAM50A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-FAM50A antibody (Cat#2066). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FAM50A antibody (Cat#2066, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    FAM50A
  • 货号:
    2066
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-FAM50A Rabbit Monoclonal Antibody
Aliases FAM50A; Family With Sequence Similarity 50 Member A; DXS9928E; XAP5; HXC-26; 9F; Protein FAM50A; Protein XAP-; HXC26; DNA Segment On Chromosome X (Unique) 9928 Expressed Sequence; Armfield X-Linked Mental Retardation Syndrome; Family With Sequence Similarity 50, Member A; Protein HXC-26; MRXSA
Background

Gene Name: FAM50A

NCBI Gene Entry: 9130

UniProt Entry: Q14320

Application Information

Molecular Weight: Predicted, 40 kDa; observed, 37 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5830

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human FAM50A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of FAM50A expression in HepG2 cells using FAM50A antibody (Cat#2066, 1:2,000). Green, isotype control; red, FAM50A.
  • Immunocytochemical staining of HepG2 cells with anti-FAM50A antibody (Cat#2066, 1:1,000). Nuclei were stained blue with DAPI; FAM50A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-FAM50A antibody (Cat#2066). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FAM50A antibody (Cat#2066, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of FAM50A expression in HepG2 cells using FAM50A antibody (Cat#2066, 1:2,000). Green, isotype control; red, FAM50A.
Immunocytochemical staining of HepG2 cells with anti-FAM50A antibody (Cat#2066, 1:1,000). Nuclei were stained blue with DAPI; FAM50A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-FAM50A antibody (Cat#2066). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FAM50A antibody (Cat#2066, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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