Anti-Lysine demethylase 1B Rabbit Monoclonal Antibody - 产品中心

Anti-Lysine demethylase 1B Rabbit Monoclonal Antibody #4376

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Flow cytometric analysis of lysine demethylase 1B expression in HepG2 cells using anti-lysine demethylase 1B antibody (Cat#4376, 1:2,000). Green, isotype control; red, lysine demethylase 1B.

Immunocytochemical staining of HepG2 cells with anti-lysine demethylase 1B antibody (Cat#4376, 1:1,000). Nuclei were stained blue with DAPI; Lysine demethylase 1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using anti-lysine demethylase 1B antibody (Cat#4376). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-lysine demethylase 1B antibody (Cat#4376, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

KDM1B
货号：

4376
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Lysine demethylase 1B Rabbit Monoclonal Antibody
Aliases	KDM1B; Lysine Demethylase 1B; LSD2; AOF1; Flavin-Containing Amine Oxidase Domain-Containing Protein 1; Lysine-Specific Histone Demethylase 1B; Lysine-Specific Histone Demethylase; Lysine (K)-Specific Demethylase 1B; DJ298J15.2; BA204B7.3; FLJ34109; FLJ33898; FLJ43328; C6orf193; Amine Oxidase (Flavin Containing) Domain 1; Chromosome 6 Open Reading Frame 193; Amine Oxidase, Flavin Containing 1; EC 1.14.99.66; C6ORF193
Background	Gene Name: KDM1B NCBI Gene Entry: 221656 UniProt Entry: Q8NB78
Application Information	Molecular Weight: Predicted, 92 kDa; observed, 75 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10910 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human LSD2 / AOF1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of lysine demethylase 1B expression in HepG2 cells using anti-lysine demethylase 1B antibody (Cat#4376, 1:2,000). Green, isotype control; red, lysine demethylase 1B.
Immunocytochemical staining of HepG2 cells with anti-lysine demethylase 1B antibody (Cat#4376, 1:1,000). Nuclei were stained blue with DAPI; Lysine demethylase 1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-lysine demethylase 1B antibody (Cat#4376). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-lysine demethylase 1B antibody (Cat#4376, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

KDM1B
货号：

4376
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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