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Anti-Glucokinase Rabbit Monoclonal Antibody #2069

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Flow cytometric analysis of Glucokinase expression in HepG2 cells using Glucokinase antibody (Cat#2069, 1:2,000). Green, isotype control; red, Glucokinase.
Immunocytochemical staining of HepG2 cells with anti-Glucokinase antibody (Cat#2069, 1:1,000). Nuclei were stained blue with DAPI; Glucokinase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-Glucokinase antibody (Cat#2069). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glucokinase antibody (Cat#2069, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    GCK
  • 货号：
    2069
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Glucokinase Rabbit Monoclonal Antibody
Aliases GCK; Glucokinase; HK4; Glucokinase (Hexokinase 4); Hexokinase Type IV; Hexokinase-4; MODY2; HK IV; Maturity Onset Diabetes Of The Young 2; ATP:D-Hexose 6-Phosphotransferase; Hexokinase D, Pancreatic Isozyme; Hexokinase 4; Hexokinase-D; EC 2.7.1.1; EC 2.7.1; FGQTL3; PNDM1; HHF3; HKIV; HXKP; LGLK; GLK; GK
Background

Gene Name: GCK

NCBI Gene Entry: 2645

UniProt Entry: P35557
Application Information

Molecular Weight: Predicted, 52 kDa; observed, 52 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5845

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Glucokinase
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Glucokinase expression in HepG2 cells using Glucokinase antibody (Cat#2069, 1:2,000). Green, isotype control; red, Glucokinase.
  • Immunocytochemical staining of HepG2 cells with anti-Glucokinase antibody (Cat#2069, 1:1,000). Nuclei were stained blue with DAPI; Glucokinase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-Glucokinase antibody (Cat#2069). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glucokinase antibody (Cat#2069, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    GCK
  • 货号：
    2069
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Glucokinase expression in HepG2 cells using Glucokinase antibody (Cat#2069, 1:2,000). Green, isotype control; red, Glucokinase.
Immunocytochemical staining of HepG2 cells with anti-Glucokinase antibody (Cat#2069, 1:1,000). Nuclei were stained blue with DAPI; Glucokinase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-Glucokinase antibody (Cat#2069). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glucokinase antibody (Cat#2069, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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