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KD-Validated Anti-DCAF7 Rabbit Monoclonal Antibody #61922

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Flow cytometric analysis of DCAF7 expression in HAP-1 cells using DCAF7 antibody (Cat#61922, 1:2,000). Green, isotype control; red, DCAF7.
Immunocytochemical staining of HAP-1 cells with anti-DCAF7 antibody (Cat#61922, 1:1,000). Nuclei were stained blue with DAPI; DCAF7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-DCAF7 antibody (Cat#61922). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DCAF7 antibody (Cat#61922, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-DCAF7 antibody (Cat#61922). DCAF7 expression in wild-type (WT) and DCAF7 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DCAF7 antibody (Cat#61922, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    DCAF7
  • 货号:
    61922
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-DCAF7 Rabbit Monoclonal Antibody
Aliases DCAF7; DDB1 And CUL4 Associated Factor 7; HAN11; SWAN-1; WDR68; Seven-WD-Repeat Protein Of The AN11 Family-1; WD Repeat-Containing Protein An11 Homolog; DDB1- And CUL4-Associated Factor 7; WD Repeat-Containing Protein 68; Human Anthocyanin; WD Repeat Domain 68; AN11
Background

Gene Name: DCAF7

NCBI Gene Entry: 10238

UniProt Entry: P61962

Application Information

Molecular Weight: Predicted, 39 kDa; observed, 39 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2235

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from DCAF7
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of DCAF7 expression in HAP-1 cells using DCAF7 antibody (Cat#61922, 1:2,000). Green, isotype control; red, DCAF7.
  • Immunocytochemical staining of HAP-1 cells with anti-DCAF7 antibody (Cat#61922, 1:1,000). Nuclei were stained blue with DAPI; DCAF7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-DCAF7 antibody (Cat#61922). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DCAF7 antibody (Cat#61922, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-DCAF7 antibody (Cat#61922). DCAF7 expression in wild-type (WT) and DCAF7 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DCAF7 antibody (Cat#61922, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of DCAF7 expression in HAP-1 cells using DCAF7 antibody (Cat#61922, 1:2,000). Green, isotype control; red, DCAF7.
Immunocytochemical staining of HAP-1 cells with anti-DCAF7 antibody (Cat#61922, 1:1,000). Nuclei were stained blue with DAPI; DCAF7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-DCAF7 antibody (Cat#61922). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DCAF7 antibody (Cat#61922, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-DCAF7 antibody (Cat#61922). DCAF7 expression in wild-type (WT) and DCAF7 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DCAF7 antibody (Cat#61922, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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