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Anti-IL12B Rabbit Monoclonal Antibody #2094

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Flow cytometric analysis of IL12B expression in HepG2 cells using IL12B antibody (Cat#2094, 1:2,000). Green, isotype control; red, IL12B.

Immunocytochemical staining of HepG2 cells with anti-IL12B antibody (Cat#2094, 1:1,000). Nuclei were stained blue with DAPI; IL12B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-IL12B antibody (Cat#2094). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIL12B antibody (Cat#2094, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

IL12B
货号：

2094
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-IL12B Rabbit Monoclonal Antibody
Aliases	IL12B; Interleukin 12B; IL-12B; CLMF2; NKSF2; CLMF; NKSF; Interleukin 12B (Natural Killer Cell Stimulatory Factor 2, Cytotoxic Lymphocyte Maturation Factor 2, P40); Natural Killer Cell Stimulatory Factor, 40 KD Subunit; Cytotoxic Lymphocyte Maturation Factor 40 KDa Subunit; NK Cell Stimulatory Factor Chain; Interleukin-12 Subunit Beta; Interleukin-12 Beta Chain; Interleukin 12, P40; IL12, Subunit P40; IL-12 Subunit P40; CLMF P40; Cytotoxic Lymphocyte Maturation Factor 2, P40; Natural Killer Cell Stimulatory Factor-2; IMD28; IMD29
Background	Gene Name: IL12B NCBI Gene Entry: 3593 UniProt Entry: P29460
Application Information	Molecular Weight: Predicted, 37 kDa; observed, 75 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5970 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human IL12B
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of IL12B expression in HepG2 cells using IL12B antibody (Cat#2094, 1:2,000). Green, isotype control; red, IL12B.
Immunocytochemical staining of HepG2 cells with anti-IL12B antibody (Cat#2094, 1:1,000). Nuclei were stained blue with DAPI; IL12B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-IL12B antibody (Cat#2094). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIL12B antibody (Cat#2094, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

IL12B
货号：

2094
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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