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KD-Validated Anti-POLDIP2 Rabbit Monoclonal Antibody #65591

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Flow cytometric analysis of POLDIP2 expression in HepG2 cells using anti-POLDIP2 antibody (Cat#65591, 1:2,000). Green, isotype control; red, POLDIP2.
Western blotting analysis using anti-POLDIP2 antibody (Cat#65591). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-POLDIP2 antibody (Cat#65591, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-POLDIP2 antibody (Cat#65591). POLDIP2 expression in wild-type (WT) and POLDIP2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-POLDIP2 antibody (Cat#65591, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with anti-POLDIP2 antibody (Cat#65591, 1:1,000) . Nuclei were stained blue with DAPI; POLDIP2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • 基因名：
    POLDIP2
  • 货号：
    65591
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-POLDIP2 Rabbit Monoclonal Antibody
Aliases POLDIP2; DNA Polymerase Delta Interacting Protein 2; PDIP38; Polymerase (DNA-Directed), Delta Interacting Protein 2; 38 KDa DNA Polymerase Delta Interaction Protein; Polymerase Delta-Interacting Protein 2; DKFZP586F1524; POLD4; P38; Polymerase (DNA) Delta Interacting Protein 2; Polymerase Delta Interacting Protein 38
Background

Gene Name: POLDIP2

NCBI Gene Entry: 26073

UniProt Entry: Q9Y2S7
Application Information

Molecular Weight: Predicted, 42 kDa; observed, 35 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4415

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human POLDIP2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of POLDIP2 expression in HepG2 cells using anti-POLDIP2 antibody (Cat#65591, 1:2,000). Green, isotype control; red, POLDIP2.
  • Western blotting analysis using anti-POLDIP2 antibody (Cat#65591). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-POLDIP2 antibody (Cat#65591, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-POLDIP2 antibody (Cat#65591). POLDIP2 expression in wild-type (WT) and POLDIP2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-POLDIP2 antibody (Cat#65591, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with anti-POLDIP2 antibody (Cat#65591, 1:1,000) . Nuclei were stained blue with DAPI; POLDIP2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Citation(0)
  • 基因名：
    POLDIP2
  • 货号：
    65591
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of POLDIP2 expression in HepG2 cells using anti-POLDIP2 antibody (Cat#65591, 1:2,000). Green, isotype control; red, POLDIP2.
Western blotting analysis using anti-POLDIP2 antibody (Cat#65591). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-POLDIP2 antibody (Cat#65591, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-POLDIP2 antibody (Cat#65591). POLDIP2 expression in wild-type (WT) and POLDIP2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-POLDIP2 antibody (Cat#65591, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with anti-POLDIP2 antibody (Cat#65591, 1:1,000) . Nuclei were stained blue with DAPI; POLDIP2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
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