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Anti-Inosine Triphosphatase Rabbit Monoclonal Antibody #2095

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Flow cytometric analysis of Inosine triphosphatase expression in HAP-1 cells using anti-Inosine triphosphatase antibody (Cat#2095, 1:2000). Green, isotype control; red, Inosine triphosphatase.
Immunocytochemical staining of HAP1 cells with anti-Inosine triphosphatase antibody (Cat #2095, 1:1,000) . Nuclei were stained blue with DAPI; Inosine triphosphatase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Inosine triphosphatase antibody (Cat#2095). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Inosine triphosphatase antibody (Cat#2095, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    ITPA
  • 货号:
    2095
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Inosine Triphosphatase Rabbit Monoclonal Antibody
Aliases ITPA; Inosine Triphosphatase; Nucleoside-Triphosphate Diphosphatase; HLC14-06-P; DJ794I6.3; Inosine Triphosphatase (Nucleoside Triphosphate Pyrophosphatase); Non-Canonical Purine NTP Pyrophosphatase; Non-Standard Purine NTP Pyrophosphatase; Inosine Triphosphate Pyrophosphatase; C20orf37; ITPase; NTPase; Epididymis Secretory Sperm Binding Protein; Inosine Triphosphate Pyrophosphohydrolase; Nucleoside-Triphosphate Pyrophosphatase; Putative Oncogene Protein HLC14-06-P; Putative Oncogene Protein Hlc14-06-P; EC 3.6.1.19; EC 3.6.1.9; C20ORF37; EC 3.6.1; DEE35; My049
Background

Gene Name: ITPA

NCBI Gene Entry: 3704

UniProt Entry: Q9BY32

Application Information

Molecular Weight: Predicted, 21 kDa; observed, 22 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5975

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human ITPA
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Inosine triphosphatase expression in HAP-1 cells using anti-Inosine triphosphatase antibody (Cat#2095, 1:2000). Green, isotype control; red, Inosine triphosphatase.
  • Immunocytochemical staining of HAP1 cells with anti-Inosine triphosphatase antibody (Cat #2095, 1:1,000) . Nuclei were stained blue with DAPI; Inosine triphosphatase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-Inosine triphosphatase antibody (Cat#2095). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Inosine triphosphatase antibody (Cat#2095, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Inosine triphosphatase expression in HAP-1 cells using anti-Inosine triphosphatase antibody (Cat#2095, 1:2000). Green, isotype control; red, Inosine triphosphatase.
Immunocytochemical staining of HAP1 cells with anti-Inosine triphosphatase antibody (Cat #2095, 1:1,000) . Nuclei were stained blue with DAPI; Inosine triphosphatase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Inosine triphosphatase antibody (Cat#2095). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Inosine triphosphatase antibody (Cat#2095, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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