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Anti-Chaperonin Containing TCP1 Subunit 2 Rabbit Monoclonal Antibody #3121

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Flow cytometric analysis of CCT2 expression in HepG2 cells using anti-CCT2 antibody (Cat#3121, 1:2,000). Green, isotype control; red, CCT2.
Immunocytochemical staining of HepG2 cells with anti-phospho-nucleophosmin (S125) antibody (Cat#3123, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Nucleophosmin (S125) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-CCT2 antibody (Cat#3121). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CCT2 antibody (Cat#3121, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CCT2
  • 货号:
    3121
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Chaperonin Containing TCP1 Subunit 2 Rabbit Monoclonal Antibody
Aliases CCT2; Chaperonin Containing TCP1 Subunit 2; Cctb; Chaperonin Containing TCP1, Subunit 2 (Beta); T-Complex Protein 1 Subunit Beta; TCP-1-Beta; CCT-Beta; 99D8.1; Chaperonin Containing T-Complex Polypeptide 1, Beta Subunit; Chaperonin Containing T-Complex Polypeptide 1, Subunit 2; Epididymis Secretory Sperm Binding Protein Li 100n; T-Complex Protein 1, Beta Subunit; HEL-S-100n; PRO1633; CCTB
Background

Gene Name: CCT2

NCBI Gene Entry: 10576

UniProt Entry: P78371

Application Information

Molecular Weight: Predicted, 57 kDa; observed, 54 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4170

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human TCP1 beta
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CCT2 expression in HepG2 cells using anti-CCT2 antibody (Cat#3121, 1:2,000). Green, isotype control; red, CCT2.
  • Immunocytochemical staining of HepG2 cells with anti-phospho-nucleophosmin (S125) antibody (Cat#3123, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Nucleophosmin (S125) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-CCT2 antibody (Cat#3121). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CCT2 antibody (Cat#3121, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of CCT2 expression in HepG2 cells using anti-CCT2 antibody (Cat#3121, 1:2,000). Green, isotype control; red, CCT2.
Immunocytochemical staining of HepG2 cells with anti-phospho-nucleophosmin (S125) antibody (Cat#3123, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Nucleophosmin (S125) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-CCT2 antibody (Cat#3121). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CCT2 antibody (Cat#3121, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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