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Anti-HLA G Rabbit Monoclonal Antibody #2147

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Immunocytochemical staining of HAP-1 cells with anti-HLA-G antibody (Cat#2147, 1:1,000) . Nuclei were stained blue with DAPI; HLA-G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-HLA-G antibody (Cat#2147). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HLA-G antibody (Cat#2147, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    HLA-G
  • 货号:
    2147
  • 应用:
    WB, IC
  • 物种反应性:
    H, M
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-HLA G Rabbit Monoclonal Antibody
Aliases HLA-G; Major Histocompatibility Complex, Class I, G; HLA Class I Histocompatibility Antigen, Alpha Chain G; HLA-G Histocompatibility Antigen, Class I, G; MHC Class I Antigen G; B2 Microglobulin; HLA G Antigen; Mutant MHC Class Ib Antigen; Mutant MHC Class I Antigen; MHC Class Ib Antigen; MHC Class I Protein; HLA-6.0; MHC-G; HLAG
Background

Gene Name: HLA-G

NCBI Gene Entry: 3135

UniProt Entry: P17693

Application Information

Molecular Weight: Predicted, 38 kDa; observed, 42 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB6225

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human HLA G
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HAP-1 cells with anti-HLA-G antibody (Cat#2147, 1:1,000) . Nuclei were stained blue with DAPI; HLA-G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-HLA-G antibody (Cat#2147). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HLA-G antibody (Cat#2147, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Immunocytochemical staining of HAP-1 cells with anti-HLA-G antibody (Cat#2147, 1:1,000) . Nuclei were stained blue with DAPI; HLA-G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-HLA-G antibody (Cat#2147). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HLA-G antibody (Cat#2147, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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