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Anti-Bridging integrator 1 Rabbit Monoclonal Antibody #4059

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Flow cytometric analysis of bridging integrator 1 expression in C2C12 cells using anti-bridging integrator 1 antibody (Cat#4059, 1:2,000). Green, isotype control; red, bridging integrator 1.

Immunocytochemical staining of C2C12 cells with anti-Bridging integrator 1 antibody (Cat#4059, 1:1,000) . Nuclei were stained blue with DAPI; Bridging integrator 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-bridging integrator 1 antibody (Cat#4059). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-bridging integrator 1 antibody (Cat#4059, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

BIN1
货号：

4059
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-Bridging integrator 1 Rabbit Monoclonal Antibody
Aliases	BIN1; Bridging Integrator 1; Myc Box-Dependent-Interacting Protein 1; Amphiphysin II; SH3P9; AMPH2; AMPHL; Box-Dependent Myc-Interacting Protein 1; Amphiphysin-Like Protein; Amphiphysin 2; Box Dependant MYC Interacting Protein 1; CNM2
Background	Gene Name: BIN1 NCBI Gene Entry: 274 UniProt Entry: O00499
Application Information	Molecular Weight: Predicted, 65 kDa; observed, 50, 60,65 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB9385 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human BIN1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of bridging integrator 1 expression in C2C12 cells using anti-bridging integrator 1 antibody (Cat#4059, 1:2,000). Green, isotype control; red, bridging integrator 1.
Immunocytochemical staining of C2C12 cells with anti-Bridging integrator 1 antibody (Cat#4059, 1:1,000) . Nuclei were stained blue with DAPI; Bridging integrator 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-bridging integrator 1 antibody (Cat#4059). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-bridging integrator 1 antibody (Cat#4059, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

BIN1
货号：

4059
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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