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Anti-HLA B Rabbit Monoclonal Antibody #2179

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Flow cytometric analysis of HLA-B expression in HAP-1 cells using anti-HLA-B antibody (Cat#2179, 1:2000). Green, isotype control; red, HLA-B.
Immunocytochemical staining of HAP1 cells with anti-HLA-B antibody (Cat #2179, 1:1,000) . Nuclei were stained blue with DAPI; HLA-B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-HLA-B antibody (Cat#2179). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HLA-B antibody (Cat#2179, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    HLA-B
  • 货号:
    2179
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-HLA B Rabbit Monoclonal Antibody
Aliases HLA-B; Major Histocompatibility Complex, Class I, B; HLA Class I Histocompatibility Antigen, B Alpha Chain; HLAB; AS; MHC Class I Antigen HLA-B Alpha Chain; MHC Class I Antigen HLA-B Heavy Chain; MHC HLA-B Transmembrane Glycoprotein; MHC HLA-B Cell Surface Glycoprotein; Leukocyte Antigen Class I-B; HLA Class I Antigen HLA-B; MHC Class I Antigen SHCHA; Human Leukocyte Antigen B; Ankylosing Spondylitis; MHC Class I Molecule; MHC Class 1 Antigen; B-4901
Background

Gene Name: HLA-B

NCBI Gene Entry: 3106

UniProt Entry: P01889

Application Information

Molecular Weight: Predicted, 40 kDa; observed, 40 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB6375

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human HLA B7
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of HLA-B expression in HAP-1 cells using anti-HLA-B antibody (Cat#2179, 1:2000). Green, isotype control; red, HLA-B.
  • Immunocytochemical staining of HAP1 cells with anti-HLA-B antibody (Cat #2179, 1:1,000) . Nuclei were stained blue with DAPI; HLA-B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-HLA-B antibody (Cat#2179). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HLA-B antibody (Cat#2179, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of HLA-B expression in HAP-1 cells using anti-HLA-B antibody (Cat#2179, 1:2000). Green, isotype control; red, HLA-B.
Immunocytochemical staining of HAP1 cells with anti-HLA-B antibody (Cat #2179, 1:1,000) . Nuclei were stained blue with DAPI; HLA-B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-HLA-B antibody (Cat#2179). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HLA-B antibody (Cat#2179, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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