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Anti-Otx1 Rabbit Monoclonal Antibody #2246

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Flow cytometric analysis of Otx1 expression in HAP-1 cells using anti-Otx1 antibody (Cat#2246, 1：2000). Green, isotype control; red, Otx1.

Immunocytochemical staining of HAP-1 cells with anti-Otx1 antibody (Cat #2246, 1:1,000) . Nuclei were stained blue with DAPI; Otx1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Scale bar, 20 μm.

Western blotting analysis using anti-Otx1 antibody (Cat#2246). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Otx1 antibody (Cat#2246, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

OTX1/2
货号：

2246
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-Otx1 Rabbit Monoclonal Antibody
Aliases	OTX1; Orthodenticle Homeobox 1; Homeobox Protein OTX1; Orthodenticle Homolog 1; Orthodenticle (Drosophila) Homolog 1; Orthodenticle Homolog 1 (Drosophila); OTX2; Orthodenticle Homeobox 2; Orthodenticle Homolog 2; Homeobox Protein OTX2; Orthodenticle Homolog 2 (Drosophila); MCOPS5; CPHD6
Background	Gene Name: OTX1/2 NCBI Gene Entry: 5013/5015 UniProt Entry: P32242/P32243
Application Information	Molecular Weight: Predicted, 32 kDa; observed, 32 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB6680 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Otx1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Otx1 expression in HAP-1 cells using anti-Otx1 antibody (Cat#2246, 1：2000). Green, isotype control; red, Otx1.
Immunocytochemical staining of HAP-1 cells with anti-Otx1 antibody (Cat #2246, 1:1,000) . Nuclei were stained blue with DAPI; Otx1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Scale bar, 20 μm.
Western blotting analysis using anti-Otx1 antibody (Cat#2246). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Otx1 antibody (Cat#2246, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

OTX1/2
货号：

2246
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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