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KD-Validated Anti-Enolase 2 Mouse Monoclonal Antibody #61823

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Western blotting analysis using anti-enolase 2 antibody (Cat#61823). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-enolase 2 antibody (Cat#61823, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-enolase 2 antibody (Cat#61823). Enolase 2 expression in wild-type (WT) and enolase 2 (ENO2) knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-enolase 2 antibody (Cat#61823, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    ENO2
  • 货号:
    61823
  • 应用:
    WB
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-Enolase 2 Mouse Monoclonal Antibody
Aliases ENO2; Enolase 2; NSE; Gamma-Enolase; 2-Phospho-D-Glycerate Hydro-Lyase; Enolase 2 (Gamma, Neuronal); Neuronal Enriched Enolase; Neuron-Specific Enolase; Neural Enolase; EC 4.2.1.11; Epididymis Secretory Protein Li 279; 2-Phospho-D-Glycerate Hydrolyase; Neuron Specific Gamma Enolase; Neurone-Specific Enolase; Neuron Specific Enolase; HEL-S-279
Background

NCBI Gene Entry: 2026

UniProt Entry: P09104

Application Information

Molecular Weight: Predicted MW:47 kDa Observed MW:47 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3900

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human Gamma-enolase
Isotype Mouse IgG2b kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:2,500
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-enolase 2 antibody (Cat#61823). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-enolase 2 antibody (Cat#61823, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-enolase 2 antibody (Cat#61823). Enolase 2 expression in wild-type (WT) and enolase 2 (ENO2) knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-enolase 2 antibody (Cat#61823, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Western blotting analysis using anti-enolase 2 antibody (Cat#61823). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-enolase 2 antibody (Cat#61823, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-enolase 2 antibody (Cat#61823). Enolase 2 expression in wild-type (WT) and enolase 2 (ENO2) knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-enolase 2 antibody (Cat#61823, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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