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Anti-CD116 Rabbit Monoclonal Antibody #2253

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Flow cytometric analysis of CD116 expression in HepG2 cells using CD116 antibody (Cat#2253, 1:2,000). Green, isotype control; red, CD116.

Immunocytochemical staining of HepG2 cells with anti-CD116 antibody (Cat#2253, 1:1,000). Nuclei were stained blue with DAPI; CD116 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-CD116 antibody (Cat#2253). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD116 antibody (Cat#2253, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CSF2RA
货号：

2253
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Citation(0)

Information

Product Name	Anti-CD116 Rabbit Monoclonal Antibody
Aliases	CSF2RA; Colony Stimulating Factor 2 Receptor Subunit Alpha; CD116; CSF2R; Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage); Granulocyte-Macrophage Colony-Stimulating Factor Receptor Subunit Alpha; Alpha-GM-CSF Receptor; GM-CSF-R-Alpha; CD116 Antigen; GMCSFR-Alpha; GMR-Alpha; AlphaGMR; CDw116; CSF2RY; Granulocyte-Macrophage Colony-Stimulating Factor Receptor Alpha Chain; Colony Stimulating Factor 2 Receptor Alpha Subunit; GM-CSF Receptor Alpha Subunit; ALPHAGMR; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; SMDP4; GMR
Background	Gene Name: CSF2RA NCBI Gene Entry: 1438 UniProt Entry: P15509
Application Information	Molecular Weight: Predicted, 46 kDa; observed, 50 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5695 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human CD116
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of CD116 expression in HepG2 cells using CD116 antibody (Cat#2253, 1:2,000). Green, isotype control; red, CD116.
Immunocytochemical staining of HepG2 cells with anti-CD116 antibody (Cat#2253, 1:1,000). Nuclei were stained blue with DAPI; CD116 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-CD116 antibody (Cat#2253). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD116 antibody (Cat#2253, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CSF2RA
货号：

2253
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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