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Anti-P4HTM Rabbit Monoclonal Antibody #2278

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Flow cytometric analysis of P4HTM expression in HepG2 cells using P4HTM antibody (Cat#2278, 1:2,000). Green, isotype control; red, P4HTM.
Immunocytochemical staining of HepG2 cells with anti-P4HTM antibody (Cat#2278, 1:1,000). Nuclei were stained blue with DAPI; P4HTM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-P4HTM antibody (Cat#2278). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-P4HTM antibody (Cat#2278, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    P4HTM
  • 货号:
    2278
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-P4HTM Rabbit Monoclonal Antibody
Aliases Prolyl 4-Hydroxylase, Transmembrane; P4H-TM; PH4; HIFPH4; EGLN4; PHD4; PH-4; Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum); Hypoxia-Inducible Factor Prolyl Hydroxylase 4; Prolyl Hydroxlase Domain-Containing 4; Transmembrane Prolyl 4-Hydroxylase; HIF-Prolyl Hydroxylase 4; FLJ20262; HIF-PH4; HPH-4; Hypoxia Inducible Factor Prolyl 4 Hydroxylase; Hypoxia-Inducible Factor Prolyl 4-Hydroxylase; P4H With Transmembrane Domain; Proline 4-Hydroxylase; EC 1.14.11.29; HIDEA
Background

Gene Name: P4HTM

NCBI Gene Entry: 54681

UniProt Entry: Q9NXG6

Application Information

Molecular Weight: Predicted, 57 kDa; observed, 47 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB100

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human HIF Prolyl Hydroxylases
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of P4HTM expression in HepG2 cells using P4HTM antibody (Cat#2278, 1:2,000). Green, isotype control; red, P4HTM.
  • Immunocytochemical staining of HepG2 cells with anti-P4HTM antibody (Cat#2278, 1:1,000). Nuclei were stained blue with DAPI; P4HTM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-P4HTM antibody (Cat#2278). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-P4HTM antibody (Cat#2278, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of P4HTM expression in HepG2 cells using P4HTM antibody (Cat#2278, 1:2,000). Green, isotype control; red, P4HTM.
Immunocytochemical staining of HepG2 cells with anti-P4HTM antibody (Cat#2278, 1:1,000). Nuclei were stained blue with DAPI; P4HTM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-P4HTM antibody (Cat#2278). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-P4HTM antibody (Cat#2278, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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