首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

  • 一抗
  • 二抗
  • 慢病毒
  • 细胞系
  • 裂解物
  • 试剂类
首页>产品中心>一抗

KD-Validated Anti-DAZ Associated Protein 1 Rabbit Monoclonal Antibody #63228

一键复制产品信息

返回列表

Flow cytometric analysis of DAZ associated protein 1 expression in HepG2 cells using anti-DAZ associated protein 1 antibody (Cat#63228, 1:2,000). Green, isotype control; red, DAZ associated protein 1.
Immunocytochemical staining of HepG2 cells with anti-DAZ associated protein 1 antibody (Cat#63228, 1:1,000) . Nuclei were stained blue with DAPI; DAZ associated protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: . Scale bar: 20 μm.
Western blotting analysis using anti-DAZ associated protein 1 antibody (Cat#63228). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DAZ associated protein 1 antibody (Cat#63228, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-DAZ associated protein 1 antibody (Cat#63228). DAZ associated protein 1 expression in wild-type (WT) and DAZ associated protein 1 (DAZAP1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DAZ associated protein 1 antibody (Cat#63228, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of DAZ associated protein 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-DAZ associated protein 1 antibody (Cat#63228, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-DAZ associated protein 1 antibody (Cat#63228, 1:1,000), Top panel: wild-type (WT); Bottom panal: DAZ associated protein 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;DAZ associated protein 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 基因名：
    DAZAP1
  • 货号：
    63228
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-DAZ Associated Protein 1 Rabbit Monoclonal Antibody
Aliases DAZAP1; DAZ Associated Protein 1; Deleted In Azoospermia-Associated Protein 1; DAZ-Associated Protein 1; MGC19907; Deleted In Azoospermia Associated Protein 1; Testicular Tissue Protein Li 50
Background

Gene Name: DAZAP1

NCBI Gene Entry: 26528

UniProt Entry: Q96EP5
Application Information

Molecular Weight: Predicted, 43 kDa, observed, 43 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4605

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen Recombinant protein of human DAZAP1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of DAZ associated protein 1 expression in HepG2 cells using anti-DAZ associated protein 1 antibody (Cat#63228, 1:2,000). Green, isotype control; red, DAZ associated protein 1.
  • Immunocytochemical staining of HepG2 cells with anti-DAZ associated protein 1 antibody (Cat#63228, 1:1,000) . Nuclei were stained blue with DAPI; DAZ associated protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: . Scale bar: 20 μm.
  • Western blotting analysis using anti-DAZ associated protein 1 antibody (Cat#63228). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DAZ associated protein 1 antibody (Cat#63228, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-DAZ associated protein 1 antibody (Cat#63228). DAZ associated protein 1 expression in wild-type (WT) and DAZ associated protein 1 (DAZAP1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DAZ associated protein 1 antibody (Cat#63228, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of DAZ associated protein 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-DAZ associated protein 1 antibody (Cat#63228, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-DAZ associated protein 1 antibody (Cat#63228, 1:1,000), Top panel: wild-type (WT); Bottom panal: DAZ associated protein 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;DAZ associated protein 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Citation(0)
  • 基因名：
    DAZAP1
  • 货号：
    63228
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of DAZ associated protein 1 expression in HepG2 cells using anti-DAZ associated protein 1 antibody (Cat#63228, 1:2,000). Green, isotype control; red, DAZ associated protein 1.
Immunocytochemical staining of HepG2 cells with anti-DAZ associated protein 1 antibody (Cat#63228, 1:1,000) . Nuclei were stained blue with DAPI; DAZ associated protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: . Scale bar: 20 μm.
Western blotting analysis using anti-DAZ associated protein 1 antibody (Cat#63228). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DAZ associated protein 1 antibody (Cat#63228, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-DAZ associated protein 1 antibody (Cat#63228). DAZ associated protein 1 expression in wild-type (WT) and DAZ associated protein 1 (DAZAP1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DAZ associated protein 1 antibody (Cat#63228, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of DAZ associated protein 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-DAZ associated protein 1 antibody (Cat#63228, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-DAZ associated protein 1 antibody (Cat#63228, 1:1,000), Top panel: wild-type (WT); Bottom panal: DAZ associated protein 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;DAZ associated protein 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 联系电话

    0551-63635518

    0551-65578998

  • 联系邮箱

    support@genuinbiotech.cn

    sales@genuinbiotech.cn

微信公众号

Copyright © 2025 合肥善本生物科技有限公司

All Rights Reserved. 皖ICP备2023000814号