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Anti-Cleaved PARP Rabbit Monoclonal Antibody #2290

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Flow cytometric analysis of Cleaved PARP expression in HeLa cells using Cleaved PARP antibody (Cat#2290, 1:2,000). Green, isotype control; red, Cleaved PARP.

Immunocytochemical staining of HeLa cells with anti-Cleaved PARP antibody (Cat#2290, 1:1,000). Nuclei were stained blue with DAPI; Cleaved PARP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Cleaved PARP antibody (Cat#2290). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cleaved PARP antibody (Cat#2290, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

PARP1
货号：

2290
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-Cleaved PARP Rabbit Monoclonal Antibody
Aliases	Poly(ADP-Ribose) Polymerase 1; ARTD1; Poly-PARP; ADPRT; PARP; PARS; PPOL; ADP-Ribosyltransferase (NAD+; Poly (ADP-Ribose) Polymerase); ADP-Ribosyltransferase Diphtheria Toxin-Like 1; Poly (ADP-Ribose) Polymerase Family, Member 1; Protein Poly-ADP-Ribosyltransferase PARP1; DNA ADP-Ribosyltransferase PARP1; NAD(+) ADP-Ribosyltransferase 1; Poly [ADP-Ribose] Polymerase 1; Poly[ADP-Ribose] Synthase 1; EC 2.4.2.30; ADPRT 1; PARP-1; ADP-Ribosyltransferase NAD(+); Poly(ADP-Ribosyl)Transferase; Poly(ADP-Ribose) Synthetase; EC 2.4.2.-; PADPRT-1; EC 2.4.2; ADPRT1
Background	Gene Name: PARP1 NCBI Gene Entry: 142 UniProt Entry: P09874
Application Information	Molecular Weight: Predicted, 113 kDa; observed, 25 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB160 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Cleaved PARP
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Cleaved PARP expression in HeLa cells using Cleaved PARP antibody (Cat#2290, 1:2,000). Green, isotype control; red, Cleaved PARP.
Immunocytochemical staining of HeLa cells with anti-Cleaved PARP antibody (Cat#2290, 1:1,000). Nuclei were stained blue with DAPI; Cleaved PARP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Cleaved PARP antibody (Cat#2290). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cleaved PARP antibody (Cat#2290, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

PARP1
货号：

2290
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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