Flow cytometric analysis of Phospho-4E BP1 (Thr46) expression in C2C12 cells using anti-Phospho-4E BP1 (Thr46) antibody (Cat#4683, 1:2,000). Green, isotype control; red, Phospho-4E BP1 (Thr46).
Immunocytochemical staining of C2C12 cells with anti-Phospho-4E BP1 (Thr46) antibody (Cat#4683, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-4E BP1 (Thr46) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-4E BP1 (Thr46) antibody (Cat#4683). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-4E BP1 (Thr46) antibody (Cat#4683, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
EIF4EBP1; Eukaryotic Translation Initiation Factor 4E Binding Protein 1; PHAS-I ; 4E-BP1; Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1; Eukaryotic Translation Initiation Factor 4E-Binding Protein 1; EIF4E-Binding Protein 1; 4EBP1; BP-1
Flow cytometric analysis of Phospho-4E BP1 (Thr46) expression in C2C12 cells using anti-Phospho-4E BP1 (Thr46) antibody (Cat#4683, 1:2,000). Green, isotype control; red, Phospho-4E BP1 (Thr46).
Immunocytochemical staining of C2C12 cells with anti-Phospho-4E BP1 (Thr46) antibody (Cat#4683, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-4E BP1 (Thr46) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-4E BP1 (Thr46) antibody (Cat#4683). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-4E BP1 (Thr46) antibody (Cat#4683, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Phospho-4E BP1 (Thr46) expression in C2C12 cells using anti-Phospho-4E BP1 (Thr46) antibody (Cat#4683, 1:2,000). Green, isotype control; red, Phospho-4E BP1 (Thr46).
Immunocytochemical staining of C2C12 cells with anti-Phospho-4E BP1 (Thr46) antibody (Cat#4683, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-4E BP1 (Thr46) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-4E BP1 (Thr46) antibody (Cat#4683). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-4E BP1 (Thr46) antibody (Cat#4683, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
