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Anti-MAP2K1/MAP2K2 Rabbit Monoclonal Antibody #2293

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Flow cytometric analysis of MEK1/2 expression in HepG2 cells using MEK1/2 antibody (Cat#2293, 1:2,000). Green, isotype control; red, MEK1/2.
Immunocytochemical staining of HepG2 cells with anti-MEK1/2 antibody (Cat#2293, 1:1,000). Nuclei were stained blue with DAPI; MEK1/2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-MEK1/2 antibody (Cat#2293). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MEK1/2 antibody (Cat#2293, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    MAP2K1/MAP2K2
  • 货号:
    2293
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-MAP2K1/MAP2K2 Rabbit Monoclonal Antibody
Aliases Mitogen-Activated Protein Kinase Kinase 1; MEK1; Dual Specificity Mitogen-Activated Protein Kinase Kinase 1; MAPK/ERK Kinase 1; MAPKK1; PRKMK1; MKK1; ERK Activator Kinase 1; MAP Kinase Kinase 1; EC 2.7.12.2; MAPKK 1; MEK 1; Protein Kinase, Mitogen-Activated, Kinase 1 (MAP Kinase Kinase 1); CFC3; MEL; Mitogen-Activated Protein Kinase Kinase 2; MEK2; MAP Kinase Kinase 2; PRKMK2; MKK2; Dual Specificity Mitogen-Activated Protein Kinase Kinase 2; ERK Activator Kinase 2; MAPK/ERK Kinase 2; EC 2.7.12.2; Mitogen-Activated Protein Kinase Kinase 2, P45; MAPKK 2; MAPKK2; MEK 2; CFC4
Background

Gene Name: MAP2K1/MAP2K2

NCBI Gene Entry: 5604/5605

UniProt Entry: Q02750/P36507

Application Information

Molecular Weight: Predicted, 43/44 kDa; observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB175

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human MEK1/2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of MEK1/2 expression in HepG2 cells using MEK1/2 antibody (Cat#2293, 1:2,000). Green, isotype control; red, MEK1/2.
  • Immunocytochemical staining of HepG2 cells with anti-MEK1/2 antibody (Cat#2293, 1:1,000). Nuclei were stained blue with DAPI; MEK1/2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-MEK1/2 antibody (Cat#2293). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MEK1/2 antibody (Cat#2293, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of MEK1/2 expression in HepG2 cells using MEK1/2 antibody (Cat#2293, 1:2,000). Green, isotype control; red, MEK1/2.
Immunocytochemical staining of HepG2 cells with anti-MEK1/2 antibody (Cat#2293, 1:1,000). Nuclei were stained blue with DAPI; MEK1/2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-MEK1/2 antibody (Cat#2293). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MEK1/2 antibody (Cat#2293, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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