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Anti-Smac/Diablo Rabbit Monoclonal Antibody #2311

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Flow cytometric analysis of Smac/Diabl expression in HepG2 cells using Smac/Diabl antibody (Cat#2311, 1:2,000). Green, isotype control; red, Smac/Diabl.
Immunocytochemical staining of HepG2 cells with Smac/Diablo antibody (Cat#2311, 1:1,000). Nuclei were stained blue with DAPI; Smac/Diablo was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Smac/Diablo antibody (Cat#2311). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Smac/Diablo antibody (Cat#2311, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    DIABLO
  • 货号：
    2311
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Smac/Diablo Rabbit Monoclonal Antibody
Aliases Diablo IAP-Binding Mitochondrial Protein; SMAC; Second Mitochondria-Derived Activator Of Caspase; DFNA64; Direct IAP-Binding Protein With Low PI; Diablo Homolog, Mitochondrial; DIABLO-S; FLJ25049; FLJ10537; Diablo, IAP-Binding Mitochondrial Protein; Diablo-Like Protein; Smac
Background

Gene Name: DIABLO

NCBI Gene Entry: 56616

UniProt Entry: Q9NR28
Application Information

Molecular Weight: Predicted, 27 kDa; observed, 21 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB800

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Smac/Diablo
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Smac/Diabl expression in HepG2 cells using Smac/Diabl antibody (Cat#2311, 1:2,000). Green, isotype control; red, Smac/Diabl.
  • Immunocytochemical staining of HepG2 cells with Smac/Diablo antibody (Cat#2311, 1:1,000). Nuclei were stained blue with DAPI; Smac/Diablo was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Smac/Diablo antibody (Cat#2311). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Smac/Diablo antibody (Cat#2311, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    DIABLO
  • 货号：
    2311
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Smac/Diabl expression in HepG2 cells using Smac/Diabl antibody (Cat#2311, 1:2,000). Green, isotype control; red, Smac/Diabl.
Immunocytochemical staining of HepG2 cells with Smac/Diablo antibody (Cat#2311, 1:1,000). Nuclei were stained blue with DAPI; Smac/Diablo was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Smac/Diablo antibody (Cat#2311). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Smac/Diablo antibody (Cat#2311, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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