Flow cytometric analysis of Versican expression in HepG2 cells using anti-Versican antibody (Cat#62725, 1:2,000). Green, isotype control; red, Versican.
Immunocytochemical staining of HepG2 cells with anti-Versican antibody (Cat#62725, 1:1,000). Nuclei were stained blue with DAPI; Versican was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Versican antibody (Cat#62725). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Versican antibody (Cat#62725, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-versican antibody (Cat#62725). Versican expression in wild-type (WT) and versican (VCAN) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-versican antibody (Cat#62725, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Versican knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with anti-Versican antibody (Cat#62725, 1:2,000) and analyzed using BD flow cytometer.
