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Anti-NRAS/HRAS/KRAS Rabbit Monoclonal Antibody #2334

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Flow cytometric analysis of Ras expression in HepG2 cells using Ras antibody (Cat#2334, 1:2,000). Green, isotype control; red, Ras.
Immunocytochemical staining of Hela cells with anti-Ras antibody (Cat#2334, 1:1,000). Nuclei were stained blue with DAPI; Ras was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Ras antibody (Cat#2334). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ras antibody (Cat#2334, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    NRAS/HRAS/KRAS
  • 货号：
    2334
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-NRAS/HRAS/KRAS Rabbit Monoclonal Antibody
Aliases NRAS Proto-Oncogene, GTPase; N-Ras; Neuroblastoma RAS Viral (V-Ras) Oncogene Homolog; Neuroblastoma RAS Viral Oncogene Homolog; Transforming Protein N-Ras; GTPase NRas; V-Ras Neuroblastoma RAS Viral Oncogene Homolog; Proto-Oncogene GTPase; N-Ras Protein Part 4; EC 3.6.5.2; ALPS4; NRAS1; HRAS1; CMNS; KRAS; NCMS; NS6; HRas Proto-Oncogene, GTPase; HRAS1; V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog; Harvey Rat Sarcoma Viral Oncogene Homolog; Transforming Protein P21; GTPase HRas; P21ras; Ras Family Small GTP Binding Protein H-Ras; Harvey Rat Sarcoma Viral Oncoprotein; Transformation Gene: Oncogene HAMSV; GTP- And GDP-Binding Peptide B; Ha-Ras1 Proto-Oncoprotein; C-Has/Bas P21 Protein; P19 H-RasIDX Protein; EC 3.6.5.2; C-BAS/HAS; C-HA-RAS1; H-RASIDX; C-H-RAS; H-Ras-1; C-H-Ras; Ha-Ras; HAMSV; RASH1; CTLO; KRAS Proto-Oncogene, GTPase; K-Ras4B; KRAS1; KRAS2; V-Ki-Ras2 Kirsten Rat Sarcoma 2 Viral Oncogene Homolog; Kirsten Rat Sarcoma Viral Oncogene Homolog; GTPase KRas; C-Ki-Ras; K-Ras 2; RASK2; V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog; Kirsten Rat Sarcoma Viral Proto-Oncogene; Cellular Transforming Proto-Oncogene; Cellular C-Ki-Ras2 Proto-Oncogene; Transforming Protein P21; PR310 C-K-Ras Oncogene; C-Kirsten-Ras Protein; Proto-Oncogene GTPase; K-Ras P21 Protein; Oncogene KRAS2; EC 3.6.5.2; C-Ki-Ras2; 'C-K-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; C-K-Ras; KI-RAS; Ki-Ras; K-Ras; CFC2; RALD; NS3; OES; NS
Background

Gene Name: NRAS/HRAS/KRAS

NCBI Gene Entry: 4893/3265/3845

UniProt Entry: P01111/P01112/P01116
Application Information

Molecular Weight: Predicted, 21 kDa; observed, 22 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB350

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Ras
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Ras expression in HepG2 cells using Ras antibody (Cat#2334, 1:2,000). Green, isotype control; red, Ras.
  • Immunocytochemical staining of Hela cells with anti-Ras antibody (Cat#2334, 1:1,000). Nuclei were stained blue with DAPI; Ras was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Ras antibody (Cat#2334). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ras antibody (Cat#2334, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    NRAS/HRAS/KRAS
  • 货号：
    2334
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Ras expression in HepG2 cells using Ras antibody (Cat#2334, 1:2,000). Green, isotype control; red, Ras.
Immunocytochemical staining of Hela cells with anti-Ras antibody (Cat#2334, 1:1,000). Nuclei were stained blue with DAPI; Ras was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Ras antibody (Cat#2334). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ras antibody (Cat#2334, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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