Flow cytometric analysis of AAMP expression in HepG2 cells using anti-AAMP antibody (Cat#69156, 1:2,000). Green, isotype control; red, AAMP.

Immunocytochemical staining of HeLa cells using anti-Angio associated migratory cell protein antibody (Cat#69156, 1:1,000), Top panel: wild-type (WT); Bottom panal: Angio associated migratory cell protein shRNA knockdown (KD). Nuclei were stained blue with DAPI;Angio associated migratory cell protein was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Western blotting analysis using anti-Angio associated migratory cell protein antibody (Cat#69156). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Angio associated migratory cell protein antibody (Cat#69156, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-Angio associated migratory cell protein antibody (Cat#69156). Angio associated migratory cell protein expression in wild type (WT) and angio associated migratory cell protein shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Angio associated migratory cell protein antibody (Cat#69156, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Immunocytochemical staining of HeLa cells using anti-Angio associated migratory cell protein antibody (Cat#69156, 1:1,000), Top panel: wild-type (WT); Bottom panal: Angio associated migratory cell protein shRNA knockdown (KD). Nuclei were stained blue with DAPI;Angio associated migratory cell protein was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.