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Anti-NDUFS2 Rabbit Monoclonal Antibody #2366

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Immunocytochemical staining of C2C12 cells with anti-NDUFS2 antibody (Cat#2366, 1:1,000). Nuclei were stained blue with DAPI; NDUFS2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NDUFS2 antibody (Cat#2366). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFS2 antibody (Cat#2366, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    NDUFS2
  • 货号:
    2366
  • 应用:
    WB, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-NDUFS2 Rabbit Monoclonal Antibody
Aliases NADH:Ubiquinone Oxidoreductase Core Subunit S2; NADH Dehydrogenase [Ubiquinone] Iron-Sulfur Protein 2, Mitochondrial; CI-49; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 2, 49kDa (NADH-Coenzyme Q Reductase); NADH-Ubiquinone Oxidoreductase 49 KDa Subunit; Complex I 49kDa Subunit; CI-49kD; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 2 (49kD) (NADH-Coenzyme Q Reductase); Complex 1, Mitochondrial Respiratory Chain, 49-KD Subunit; NADH-Ubiquinone Oxidoreductase NDUFS2 Subunit; Complex I-49kD; EC 1.6.99.5; EC 7.1.1.2; LHONAR2; MC1DN6
Background

Gene Name: NDUFS2

NCBI Gene Entry: 4720

UniProt Entry: O75306

Application Information

Molecular Weight: Predicted, 53 kDa; observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB495

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human NDUFS2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of C2C12 cells with anti-NDUFS2 antibody (Cat#2366, 1:1,000). Nuclei were stained blue with DAPI; NDUFS2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-NDUFS2 antibody (Cat#2366). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFS2 antibody (Cat#2366, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Immunocytochemical staining of C2C12 cells with anti-NDUFS2 antibody (Cat#2366, 1:1,000). Nuclei were stained blue with DAPI; NDUFS2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NDUFS2 antibody (Cat#2366). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFS2 antibody (Cat#2366, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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