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Anti-PCYT1A Rabbit Monoclonal Antibody #4704

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Flow cytometric analysis of PCYT1A expression in HepG2 cells using anti-PCYT1A antibody (Cat#4704, 1:2,000). Green, isotype control; red, PCYT1A.
Immunocytochemical staining of HepG2 cells with anti-PCYT1A antibody (Cat#4704, 1:1,000) . Nuclei were stained blue with DAPI; PCYT1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-PCYT1A antibody (Cat#4704). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PCYT1A antibody (Cat#4704, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    PCYT1A
  • 货号:
    4704
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-PCYT1A Rabbit Monoclonal Antibody
Aliases PCYT1A; Phosphate Cytidylyltransferase 1A, Choline;
CTPCT; CCTalpha; PCYT1; CT; Phosphate Cytidylyltransferase 1, Choline, Alpha; CTP:Phosphocholine Cytidylyltransferase-Alpha; Choline-Phosphate Cytidylyltransferase Alpha; CTP:Phosphocholine Cytidylyltransferase A; Choline-Phosphate Cytidylyltransferase A; Phosphorylcholine Transferase Alpha;
Phosphorylcholine Transferase A; EC 2.7.7.15 4; CCT-Alpha; CCT A; CT A;
Phosphate Cytidylyltransferase 1, Choline, Alpha Isoform; EC 2.7.7; SMDCRD; CCTA; CGL5;CTA
Background

Gene Name: PCYT1A

NCBI Gene Entry: 5130

UniProt Entry: P49585

Application Information

Molecular Weight: Predicted, 42 kDa; observed, 42 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12530

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human CT A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PCYT1A expression in HepG2 cells using anti-PCYT1A antibody (Cat#4704, 1:2,000). Green, isotype control; red, PCYT1A.
  • Immunocytochemical staining of HepG2 cells with anti-PCYT1A antibody (Cat#4704, 1:1,000) . Nuclei were stained blue with DAPI; PCYT1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-PCYT1A antibody (Cat#4704). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PCYT1A antibody (Cat#4704, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of PCYT1A expression in HepG2 cells using anti-PCYT1A antibody (Cat#4704, 1:2,000). Green, isotype control; red, PCYT1A.
Immunocytochemical staining of HepG2 cells with anti-PCYT1A antibody (Cat#4704, 1:1,000) . Nuclei were stained blue with DAPI; PCYT1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-PCYT1A antibody (Cat#4704). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PCYT1A antibody (Cat#4704, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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