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Anti-NDUFB4 Rabbit Monoclonal Antibody #2367

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Immunocytochemical staining of HeLa cells with anti-NDUFB4 antibody (Cat#2367, 1:1,000). Nuclei were stained blue with DAPI; NDUFB4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NDUFB4 antibody (Cat#2367). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB4 antibody (Cat#2367, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    NDUFB4
  • 货号:
    2367
  • 应用:
    WB, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-NDUFB4 Rabbit Monoclonal Antibody
Aliases NADH:Ubiquinone Oxidoreductase Subunit B4; B15; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 4, 15kDa; NADH Dehydrogenase [Ubiquinone] 1 Beta Subcomplex Subunit 4; NADH-Ubiquinone Oxidoreductase B15 Subunit; Complex I B15 Subunit; Complex I-B15; CI-B15; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 4 (15kD, B15)
Background

Gene Name: NDUFB4

NCBI Gene Entry: 4710

UniProt Entry: O95168

Application Information

Molecular Weight: Predicted, 15 kDa; observed, 15 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB500

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human NDUFB4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HeLa cells with anti-NDUFB4 antibody (Cat#2367, 1:1,000). Nuclei were stained blue with DAPI; NDUFB4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-NDUFB4 antibody (Cat#2367). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB4 antibody (Cat#2367, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Immunocytochemical staining of HeLa cells with anti-NDUFB4 antibody (Cat#2367, 1:1,000). Nuclei were stained blue with DAPI; NDUFB4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NDUFB4 antibody (Cat#2367). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB4 antibody (Cat#2367, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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