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Anti-MICA Rabbit Monoclonal Antibody #2396

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Immunocytochemical staining of HepG2 cells with anti-MICA antibody (Cat#2396, 1:1,000). Nuclei were stained blue with DAPI; MICA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-MICA antibody (Cat#2396). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MICA antibody (Cat#2396, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    MICA
  • 货号:
    2396
  • 应用:
    WB, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-MICA Rabbit Monoclonal Antibody
Aliases MHC Class I Polypeptide-Related Sequence A; PERB11.1; MIC-A; Major Histocompatibility Complex Class I Chain-Related Protein A; MHC Class I Chain-Related Protein A; Stress Inducible Class I Homolog; MHC Class I Related Sequence A; MHC Class I Related Chain A; HLA Class I Antigen
Background

Gene Name: MICA

NCBI Gene Entry: 100507436

UniProt Entry: Q29983

Application Information

Molecular Weight: Predicted, 43 kDa; observed, 42-70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB645

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human MICA
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HepG2 cells with anti-MICA antibody (Cat#2396, 1:1,000). Nuclei were stained blue with DAPI; MICA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-MICA antibody (Cat#2396). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MICA antibody (Cat#2396, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Immunocytochemical staining of HepG2 cells with anti-MICA antibody (Cat#2396, 1:1,000). Nuclei were stained blue with DAPI; MICA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-MICA antibody (Cat#2396). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MICA antibody (Cat#2396, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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