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KD-Validated Anti-FAS Rabbit Monoclonal Antibody #61981

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Western blotting analysis using anti-FAS antibody (Cat#61981). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FAS antibody (Cat#61981, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-FAS antibody (Cat#61981). FAS expression in wild-type (WT) and FAS shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-FAS antibody (Cat#61981, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Validation of FAS knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with anti-FAS antibody (Cat#61981, 1:2,000) and analyzed using BD flow cytometer.

基因名：

FAS
货号：

61981
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

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Information

Product Name	KD-Validated Anti-FAS Rabbit Monoclonal Antibody
Aliases	FAS; Fas Cell Surface Death Receptor; TNFRSF6; APO-1; CD95; APT1; FAS1; Tumor Necrosis Factor Receptor Superfamily, Member 6; Tumor Necrosis Factor Receptor Superfamily Member 6; Fas (TNF Receptor Superfamily, Member 6); Apoptosis-Mediating Surface Antigen FAS; TNF Receptor Superfamily Member 6; FASLG Receptor; CD95 Antigen; Mutant Tumor Necrosis Receptor Superfamily Member 6; Apoptosis Signaling Receptor FAS; APO-1 Cell Surface Antigen; Apoptosis Antigen 1; Apo-1 Antigen; Fas AMA; ALPS1A; FASTM
Background	Gene Name: FAS NCBI Gene Entry: 355 UniProt Entry: P25445
Application Information	Molecular Weight: Predicted, 38 kDa; observed, 40-50 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB6805 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human Fas
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Western blotting analysis using anti-FAS antibody (Cat#61981). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FAS antibody (Cat#61981, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-FAS antibody (Cat#61981). FAS expression in wild-type (WT) and FAS shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-FAS antibody (Cat#61981, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of FAS knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with anti-FAS antibody (Cat#61981, 1:2,000) and analyzed using BD flow cytometer.

Citation(0)

基因名：

FAS
货号：

61981
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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