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Anti-Membrane Metalloendopeptidase Rabbit Monoclonal Antibody #2409

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Flow cytometric analysis of CD10 expression in HAP-1 cells using anti-CD10 antibody (Cat#2409, 1:2,000). Green, isotype control; red, CD10.
Immunocytochemical staining of HAP1 cells with anti-CD10 antibody (Cat#2409, 1:1,000) . Nuclei were stained blue with DAPI; CD10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low. Scale bar, 20 μm.
Western blotting analysis using anti-CD10 antibody (Cat#2409). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD10 antibody (Cat#2409, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    MME
  • 货号:
    2409
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Membrane Metalloendopeptidase Rabbit Monoclonal Antibody
Aliases Membrane Metalloendopeptidase; CALLA; NEP; Neprilysin; CD10; Common Acute Lymphocytic Leukemia Antigen; Neutral Endopeptidase 24.11; Skin Fibroblast Elastase; Neutral Endopeptidase; Atriopeptidase; Enkephalinase; EC 3.4.24.11; SFE; Membrane Metallo-Endopeptidase (Neutral Endopeptidase, Enkephalinase, CALLA, CD10); Neprilysin-390; Neprilysin-411; CD10 Antigen; EC 3.4.24; CMT2T; SCA43; EPN
Background

Gene Name: MME

NCBI Gene Entry: 4311

UniProt Entry: P08473

Application Information

Molecular Weight: Predicted, 86 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB710

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human CD10
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CD10 expression in HAP-1 cells using anti-CD10 antibody (Cat#2409, 1:2,000). Green, isotype control; red, CD10.
  • Immunocytochemical staining of HAP1 cells with anti-CD10 antibody (Cat#2409, 1:1,000) . Nuclei were stained blue with DAPI; CD10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-CD10 antibody (Cat#2409). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD10 antibody (Cat#2409, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of CD10 expression in HAP-1 cells using anti-CD10 antibody (Cat#2409, 1:2,000). Green, isotype control; red, CD10.
Immunocytochemical staining of HAP1 cells with anti-CD10 antibody (Cat#2409, 1:1,000) . Nuclei were stained blue with DAPI; CD10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low. Scale bar, 20 μm.
Western blotting analysis using anti-CD10 antibody (Cat#2409). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD10 antibody (Cat#2409, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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