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Anti-RAD18 Rabbit Monoclonal Antibody #2446

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Flow cytometric analysis of RAD18 expression in H9c2 cells using anti-RAD18 antibody (Cat#2446, 1:2,000). Green, isotype control; red, RAD18.

Immunocytochemical staining of H9c2 cells with anti-RAD18 antibody (Cat#2446, 1:1,000). Nuclei were stained blue with DAPI; RAD18 was stained magenta with Alexa Fluor庐 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 渭m.

Western blotting analysis using anti-RAD18 antibody (Cat#2446). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAD18 antibody (Cat#2446, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

RAD18
货号：

2446
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-RAD18 Rabbit Monoclonal Antibody
Aliases	RAD18 E3 Ubiquitin Protein Ligase; RNF73; RING-Type E3 Ubiquitin Transferase RAD18; Postreplication Repair Protein RAD18; E3 Ubiquitin-Protein Ligase RAD18; RING Finger Protein 73; HRAD18; HHR18; Postreplication Repair Protein HRAD18p; RAD18 Homolog (S. Cerevisiae); RAD18, S. Cerevisiae, Homolog; RAD18 Homolog; EC 2.3.2.27
Background	Gene Name: RAD18 NCBI Gene Entry: 56852 UniProt Entry: Q9NS91
Application Information	Molecular Weight: Predicted, 56 kDa; observed, 70 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB880 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human RAD18
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of RAD18 expression in H9c2 cells using anti-RAD18 antibody (Cat#2446, 1:2,000). Green, isotype control; red, RAD18.
Immunocytochemical staining of H9c2 cells with anti-RAD18 antibody (Cat#2446, 1:1,000). Nuclei were stained blue with DAPI; RAD18 was stained magenta with Alexa Fluor庐 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 渭m.
Western blotting analysis using anti-RAD18 antibody (Cat#2446). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAD18 antibody (Cat#2446, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

RAD18
货号：

2446
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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