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Anti-PKC alpha + beta + gamma Rabbit Monoclonal Antibody #2447

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Flow cytometric analysis of PKC alpha + beta + gamma expression in C2C12 cells using anti-PKC alpha + beta + gamma antibody (Cat#2447, 1:2,000). Green, isotype control; red, PKC alpha + beta + gamma.
Immunocytochemical staining of C2C12 cells with anti-PKC alpha + beta + gamma antibody (Cat#2447, 1:1,000). Nuclei were stained blue with DAPI; PKC alpha + beta + gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-PKC alpha + beta + gamma antibody (Cat#2447). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PKC alpha + beta + gamma antibody (Cat#2447, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    PRKCB/PRKCA/PRKCG/PRKCE
  • 货号:
    2447
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-PKC alpha + beta + gamma Rabbit Monoclonal Antibody
Aliases Protein Kinase C Beta; PRKCB1; PKCB; Protein Kinase C Beta Type; EC 2.7.11.13; PKC-Beta; PRKCB2; PKC-B; Protein Kinase C, Beta 1 Polypeptide; Protein Kinase C, Beta 1; Protein Kinase C, Beta; EC 2.7.11; PKCI(2); PKCbeta; PKCβ; GeneCards Symbol: PRKCA; Protein Kinase C Alpha; PKCA; Protein Kinase C Alpha Type; EC 2.7.11.13; PKC-Alpha; PRKACA; PKC-A; Protein Kinase C, Alpha; Aging-Associated Gene 6; EC 2.7.11; PKCalpha; PKCI+/-; PKCα; AAG6; Protein Kinase C Gamma; PKC-Gamma; PKCC; PKCG; Protein Kinase C Gamma Type; EC 2.7.11.13; MGC57564; SCA14; Protein Kinase C, Gamma; EC 2.7.11; PKCgamma; PKCI(3); PKCγ; Protein Kinase C Epsilon; Protein Kinase C Epsilon Type; NPKC-Epsilon; EC 2.7.11.13; PKCE; Protein Kinase C, Epsilon; EC 2.7.11
Background

Gene Name: PRKCB/PRKCA/PRKCG/PRKCE

NCBI Gene Entry: 5579/5578/5582/5581

UniProt Entry: P05771/P17252/P05129/Q02156

Application Information

Molecular Weight: Predicted, 77,78 kDa; observed, 77 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB885

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human PKC
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PKC alpha + beta + gamma expression in C2C12 cells using anti-PKC alpha + beta + gamma antibody (Cat#2447, 1:2,000). Green, isotype control; red, PKC alpha + beta + gamma.
  • Immunocytochemical staining of C2C12 cells with anti-PKC alpha + beta + gamma antibody (Cat#2447, 1:1,000). Nuclei were stained blue with DAPI; PKC alpha + beta + gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-PKC alpha + beta + gamma antibody (Cat#2447). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PKC alpha + beta + gamma antibody (Cat#2447, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of PKC alpha + beta + gamma expression in C2C12 cells using anti-PKC alpha + beta + gamma antibody (Cat#2447, 1:2,000). Green, isotype control; red, PKC alpha + beta + gamma.
Immunocytochemical staining of C2C12 cells with anti-PKC alpha + beta + gamma antibody (Cat#2447, 1:1,000). Nuclei were stained blue with DAPI; PKC alpha + beta + gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-PKC alpha + beta + gamma antibody (Cat#2447). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PKC alpha + beta + gamma antibody (Cat#2447, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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