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Anti-Ring-Box 1 Rabbit Monoclonal Antibody #2461

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Flow cytometric analysis of Ring-box 1 expression in H9c2 cells using anti-Ring-box 1 antibody (Cat#2461, 1:2,000). Green, isotype control; red, Ring-box 1.
Immunocytochemical staining of H9C2 cells with anti-Ring-box 1 antibody (Cat#2461, 1:1,000) . Nuclei were stained blue with DAPI;Ring-box 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-Ring-box 1 antibody (Cat#2461). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ring-box 1 antibody (Cat#2461, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    RBX1
  • 货号：
    2461
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Ring-Box 1 Rabbit Monoclonal Antibody
Aliases Ring-Box 1; RNF75; ROC1; Regulator Of Cullins 1; BA554C12.1; E3 Ubiquitin-Protein Transferase RBX1; E3 Ubiquitin-Protein Ligase RBX1; RING Finger Protein 75; RING-Box Protein 1; Ring-Box 1, E3 Ubiquitin Protein Ligase; ZYP Protein; Protein ZYP; EC 2.3.2.27; EC 2.3.2.32; Rbx1
Background

Gene Name: RBX1

NCBI Gene Entry: 9978

UniProt Entry: P62877
Application Information

Molecular Weight: Predicted, 12 kDa; observed, 15 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB950

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human ROC1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Ring-box 1 expression in H9c2 cells using anti-Ring-box 1 antibody (Cat#2461, 1:2,000). Green, isotype control; red, Ring-box 1.
  • Immunocytochemical staining of H9C2 cells with anti-Ring-box 1 antibody (Cat#2461, 1:1,000) . Nuclei were stained blue with DAPI;Ring-box 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-Ring-box 1 antibody (Cat#2461). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ring-box 1 antibody (Cat#2461, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    RBX1
  • 货号：
    2461
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Ring-box 1 expression in H9c2 cells using anti-Ring-box 1 antibody (Cat#2461, 1:2,000). Green, isotype control; red, Ring-box 1.
Immunocytochemical staining of H9C2 cells with anti-Ring-box 1 antibody (Cat#2461, 1:1,000) . Nuclei were stained blue with DAPI;Ring-box 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-Ring-box 1 antibody (Cat#2461). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ring-box 1 antibody (Cat#2461, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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