首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

  • 一抗
  • 二抗
  • 慢病毒
  • 细胞系
  • 裂解物
  • 试剂类
首页>产品中心>一抗

KD-Validated Anti-NDUFB2 Rabbit Monoclonal Antibody #65582

一键复制产品信息

返回列表

Western blotting analysis using anti-NDUFB2 antibody (Cat#65582). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NDUFB2 antibody (Cat#65582). NDUFB2 expression in wild-type (WT) and NDUFB2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with anti-NDUFB2 antibody (Cat#65582, 1:1,000) . Nuclei were stained blue with DAPI; NDUFB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NDUFB2 antibody (Cat#65582). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NDUFB2 antibody (Cat#65582). NDUFB2 expression in wild-type (WT) and NDUFB2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of NDUFB2 expression in C2C12 cells using anti-NDUFB2 antibody (Cat#65582, 1:2,000). Green, isotype control; red, NDUFB2.
  • 基因名：
    NDUFB2
  • 货号：
    65582
  • 应用：
    WB, IC, FCM, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-NDUFB2 Rabbit Monoclonal Antibody
Aliases NDUFB2; NADH:Ubiquinone Oxidoreductase Subunit B2; CI-AGGG; NADH-Ubiquinone Oxidoreductase AGGG Subunit; AGGG; NADH Dehydrogenase [Ubiquinone] 1 Beta Subcomplex Subunit 2, Mitochondrial; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 2, 8kDa; Complex I AGGG Subunit; Complex I-AGGG; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 2 (8kD, AGGG)
Background

Gene Name: NDUFB2

NCBI Gene Entry: 4708

UniProt Entry: O95178
Application Information

Molecular Weight: Predicted, 12 kDa; observed, 12 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4370

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen Recombinant protein of human NDUFB2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-NDUFB2 antibody (Cat#65582). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-NDUFB2 antibody (Cat#65582). NDUFB2 expression in wild-type (WT) and NDUFB2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with anti-NDUFB2 antibody (Cat#65582, 1:1,000) . Nuclei were stained blue with DAPI; NDUFB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-NDUFB2 antibody (Cat#65582). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-NDUFB2 antibody (Cat#65582). NDUFB2 expression in wild-type (WT) and NDUFB2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of NDUFB2 expression in C2C12 cells using anti-NDUFB2 antibody (Cat#65582, 1:2,000). Green, isotype control; red, NDUFB2.
Citation(0)
  • 基因名：
    NDUFB2
  • 货号：
    65582
  • 应用：
    WB, IC, FCM, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Western blotting analysis using anti-NDUFB2 antibody (Cat#65582). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NDUFB2 antibody (Cat#65582). NDUFB2 expression in wild-type (WT) and NDUFB2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with anti-NDUFB2 antibody (Cat#65582, 1:1,000) . Nuclei were stained blue with DAPI; NDUFB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NDUFB2 antibody (Cat#65582). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NDUFB2 antibody (Cat#65582). NDUFB2 expression in wild-type (WT) and NDUFB2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of NDUFB2 expression in C2C12 cells using anti-NDUFB2 antibody (Cat#65582, 1:2,000). Green, isotype control; red, NDUFB2.
  • 联系电话

    0551-63635518

    0551-65578998

  • 联系邮箱

    support@genuinbiotech.cn

    sales@genuinbiotech.cn

微信公众号

Copyright © 2025 合肥善本生物科技有限公司

All Rights Reserved. 皖ICP备2023000814号