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Anti-PNKP Rabbit Monoclonal Antibody #2464

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Flow cytometric analysis of PNKP expression in HT-1080 cells using anti-PNKP antibody (Cat#2464, 1:2,000). Green, isotype control; red, PNKP.

Immunocytochemical staining of HT-1080 cells with anti-PNKP antibody (Cat#2464, 1:1,000) . Nuclei were stained blue with DAPI; PNKP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-PNKP antibody (Cat#2464). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PNKP antibody (Cat#2464, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

PNKP
货号：

2464
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-PNKP Rabbit Monoclonal Antibody
Aliases	Polynucleotide Kinase 3'-Phosphatase; PNK; Bifunctional Polynucleotide Phosphatase/Kinase; DNA 5'-Kinase/3'-Phosphatase; Homo Sapiens Polynucleotide Kinase 3'-Phosphatase (PNKP); Polynucleotide Kinase-3'-Phosphatase; CMT2B2; EIEE10; AOA4; MCSZ
Background	Gene Name: PNKP NCBI Gene Entry: 11284 UniProt Entry: Q96T60
Application Information	Molecular Weight: Predicted, 58 kDa; observed, 57 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB965 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PNK / PNKP
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PNKP expression in HT-1080 cells using anti-PNKP antibody (Cat#2464, 1:2,000). Green, isotype control; red, PNKP.
Immunocytochemical staining of HT-1080 cells with anti-PNKP antibody (Cat#2464, 1:1,000) . Nuclei were stained blue with DAPI; PNKP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PNKP antibody (Cat#2464). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PNKP antibody (Cat#2464, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

PNKP
货号：

2464
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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